DOI: 10.18129/B9.bioc.amplican    

Automated analysis of CRISPR experiments.

Bioconductor version: Release (3.6)

`amplican` performs alignment of the amplicon reads, normalizes gathered data, calculates multiple statistics (e.g. cut rates, frameshifts) and presents results in form of aggregated reports. Data and statistics can be broken down by experiments, barcodes, user defined groups, guides and amplicons allowing for quick identification of potential problems.

Author: Kornel Labun [aut], Eivind Valen [cph, cre]

Maintainer: Eivind Valen <eivind.valen at>

Citation (from within R, enter citation("amplican")):


To install this package, start R and enter:

## try http:// if https:// URLs are not supported


HTML R Script amplican overview
HTML R Script example amplicon_report report
HTML R Script example barcode_report report
HTML R Script example group_report report
HTML R Script example guide_report report
HTML R Script example id_report report
HTML R Script example index report
PDF   Reference Manual
Text   NEWS


biocViews Alignment, CRISPR, Software, Technology, qPCR
Version 1.0.0
License GPL-3
Depends R (>= 3.4.0), methods, BiocGenerics(>= 0.22.0), Biostrings(>= 2.44.2), data.table (>= 1.10.4)
Imports utils (>= 3.4.1), S4Vectors(>= 0.14.3), ShortRead(>= 1.34.0), IRanges(>= 2.10.2), GenomicRanges(>= 1.28.4), GenomeInfoDb(>= 1.12.2), BiocParallel(>= 1.10.1), gtable (>= 0.2.0), gridExtra (>= 2.2.1), ggplot2 (>= 2.2.0), ggbio(>= 1.24.1), ggthemes (>= 3.4.0), waffle (>= 0.7.0), stringr (>= 1.2.0), stats (>= 3.4.1), matrixStats (>= 0.52.2), Matrix (>= 1.2-10), dplyr (>= 0.7.2), rmarkdown (>= 1.6), knitr (>= 1.16), clusterCrit (>= 1.2.7)
Suggests testthat, BiocStyle
Depends On Me
Imports Me
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Source Package amplican_1.0.0.tar.gz
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