Template Data

Template data can be loaded in two ways. For immunological applications you can directly load IMGT template sequences. In this case, the allowed binding regions are automatically set to the leader regions and no additional adjustments need to be made.

For other application scenarios, you can upload your template sequences (in 5' to 3' direction) either as a FASTA or CSV (downloaded from the tool) file.

When inputting templates as a FASTA File, you should consider the following parameters:

Allowed binding regions

To define the primer target binding regions, you can either provide template-specific (individual) binding regions or uniform binding regions. Individual binding regions are supplied by providing FASTA files with the template-specific regions where forward and reverse primers should bind.

To specify a binding range that is identical for all template sequences, you can simply select a positional range in the templates where primer binding should occur. Note that the positional range has to be provided relative to the 5' end of the templates and relative to the 3' end of templates for forward and reverse primers, respectively.

In case that you you want to also consider binding events outside the target region (off-target binding), you can adjust allowed Off-target binding ratio to a non-zero value in the settings before starting an analysis.