Protein Significance Analysis in DDA, SRM and DIA for Label-free or Label-based Proteomics Experiments


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Documentation for package ‘MSstats’ version 3.3.11

Help Pages

MSstats-package Tools for protein significance analysis in DDA,SRM and DIA proteomic experiments for label-free workflows or workflows with stable isotope labeled reference
dataProcess Data pre-processing and quality control of MS runs of raw data
dataProcessPlots Visualization for explanatory data analysis
DDARawData Example dataset from a label-free DDA, a controlled spike-in experiment.
DDARawData.Skyline Example dataset from a label-free DDA, a controlled spike-in experiment, processed by Skyline.
designSampleSize Planning future experimental designs of Selected Reaction Monitoring (SRM), Data-Dependent Acquisition (DDA or shotgun), and Data-Independent Acquisition (DIA or SWATH-MS) experiments in sample size calculation
designSampleSizePlots Visualization for sample size calculation
DIARawData Example dataset from a label-free DIA, a group comparison study of S.Pyogenes.
groupComparison Finding differentially abundant proteins across conditions in targeted Selected Reaction Monitoring (SRM), Data-Dependent Acquisition (DDA or shotgun), and Data-Independent Acquisition (DIA or SWATH-MS) experiment
groupComparisonPlots Visualization for model-based analysis and summarizing differentially abundant proteins
linear_quantlim Calculation of the LOD and LOQ with a linear fit
MaxQtoMSstatsFormat Generate MSstats required input format for MaxQuant output
modelBasedQCPlots Visualization for model-based quality control in fitting model
MSstats Tools for protein significance analysis in DDA,SRM and DIA proteomic experiments for label-free workflows or workflows with stable isotope labeled reference
nonlinear_quantlim Calculation of the LOD and LOQ with a nonlinear fit
plot_quantlim Plot of the curve used to calculate LOD and LOQ
quantification Protein sample quantification or group quantification
SpikeInDataLinear Example dataset from an MRM spike-in experiment with a linear behavior
SpikeInDataNonLinear Example dataset from an MRM spike-in experiment with a nonlinear behavior
SRMRawData Example dataset from a SRM experiment with stable isotope labeled reference of a time course yeast study
transformMSnSetToMSstats Transforms a MSnSet class dataset into a required input for MSstats
transformMSstatsToMSnSet Transformation input format for MSstats to MSnSet class