Identifying the Frequency of Polyfunctional Antigen-Specific T cells in ICS Flow Cytometry Data

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Documentation for package ‘polyICSFlow’ version 0.99.6

• DESCRIPTION file
• User guides, package vignettes and other documentation
• Package NEWS
• Package README

Help Pages

assignMarkerCombinations	Assing each cell to a combination of gated markers.
calcPolyfunctionality	Calculate the frequency of polyfunctional cells
extractCellCounts	Get number of cells positive for each distinct marker combination
getMarkerPositivity	Create dataframe of marker positivity
plotCellCountsAtLeast	Plot counts of cells expressing at least 1, 2, ..., n markers.
plotCellCountsExactly	Plot counts of cells expressing exactly 1, 2, ..., n markers.
plotMarkerComb2DScatters	Plot 2D scatterplots of all marker combinations.
plotMarkerCombHeatmap	Plot a heatmap of all possible marker combinations.