Report breakdown by barcode
ampliCan
14 April 2026
1 Description
Read distribution plot - plot shows number of reads assigned during read grouping
Filtered Reads - plot shows percentage of assigned reads that have been recognized as PRIMER DIMERS or filtered based on low alignment score
Edit rates - plot gives overview of percentage of reads (not filtered as PRIMER DIMER) that have edits
Frameshift - plot shows what percentage of reads that have frameshift
Top unassigned reads - take a look at the alignment of most abundant forward and reverse complemented reverse reads for each barcode, if you find that there is many unassigned reads you can ivestigate here.
2 Barcode Summary
2.1 Groups IDs
| group | IDs |
|---|---|
| barcode_1 | ID_1, ID_2 |
| barcode_2 | ID_3, ID_4, ID_5 |
2.2 Read distribution
2.3 Filtered Reads
2.4 Edit rates
2.5 Frameshift
3 Top unassigned reads
3.1 barcode_1
| Forward | Reverse | Counts | Frequency |
|---|---|---|---|
| P1 | S1 | 1 | 0.0588235 |
| P2 | S2 | 1 | 0.0588235 |
| P3 | S3 | 1 | 0.0588235 |
| P4 | S4 | 1 | 0.0588235 |
P1 1 AAATACTGTCTTGTGACCAAACCTTCTTAAGGTGCTGTTTTGATGATAAA 50
|| ||| | | | ||| | || ||| |||
S1 1 AAG--CTGAC----GGCTAAA---TGAAAAATATCTG----------AAA 31
P1 51 CTTTATTGTGCTTTTGTAGTTGTGCCCCTTGTGTT--GGCAGAG-----G 93
| | || || || |||| | | || ||||||| |
S1 32 CAT-------CTGTTCCAG--GTGCTGCGTATGCCAGGGCAGAGAAGAAG 72
P1 94 GTCAGC--AGACCAGT--AAGTCTTCTCAATTTCTTTTATTT----ATGT 135
||||| || || | | ||| || | ||| || |
S1 73 GTCAGGGAAGGTCACTGGAGGTCACTGGGATACCCTTTCTTCCCACACCA 122
P1 136 AT-----ATGTAGT-------GATAAA-A 151
|| | | ||| ||| | |
S1 123 ATGGGGAAAGGAGTCCTGCCAGATGACCA 151
P2 1 AAATACTGTCTTGTGACCAAACCTTCTTAAGGTGCTG---TTTTGATGAT 47
| || | || | | | | ||| | || || ||
S2 1 --------TTTTATCACTACATATACATAAATAAAAGAAATTGAGAAGAC 42
P2 48 NNNCTTTATTGTGCTTTTGTAGTTGTGCCCCTTGTGTTGGCAGAGGGTCA 97
||| || || || | |||| |||| | ||
S2 43 -----TTACTGGTCTGCTGACCCTCTGCCAACACAAGGGGCACAACTACA 87
P2 98 GCAGACCAGTAAG-----TCTTCTCAATTTCTTTTATTTATGTATATGTA 142
|| || ||| || || || | | || | | | |
S2 88 AAAGCACAATAAAGNNNATCATCAAAACAGCACCT---TAAGAAGGTTTG 134
P2 143 GTGATAAAA-------- 151
|| | || |
S2 135 GTCACAAGACAGTATTT 151
P3 1 AAGCTGACGGCTAAATNNNAAATATCTGAAACATCTGTTCCAGGTGCTGC 50
|||||||||||||||| |||||||||||||||||||||||||||||||
S3 1 AAGCTGACGGCTAAATGAAAAATATCTGAAACATCTGTTCCAGGTGCTGC 50
P3 51 GTATGCCAGGGCAGAGAAGAAGGTCAGGGAAGGTCACTGGAGGTCACTGG 100
||||||||||||||||||||||||||||||||||||||||||||||||||
S3 51 GTATGCCAGGGCAGAGAAGAAGGTCAGGGAAGGTCACTGGAGGTCACTGG 100
P3 101 GATACCCTTTCTTCCCACACCAATGGGGAAAGGAGTCCTGCCAGATGACC 150
||||||||||||||||||||||||||||||||||||||||||||||||||
S3 101 GATACCCTTTCTTCCCACACCAATGGGGAAAGGAGTCCTGCCAGATGACC 150
P3 151 A 151
|
S3 151 A 151
P4 1 AAGCTNGCGGCTAAATGAAAAATATCTGAAACATCTGTTCCAGGTGCTGC 50
|| |||||||||||||||||||||||||||||||||||||||||||
S4 1 NNNCTGACGGCTAAATGAAAAATATCTGAAACATCTGTTCCAGGTGCTGC 50
P4 51 GTATGCCAGGGCAGAGAAGAAGGTCAGGGAAGGTCACTGGAGGTCACTGG 100
||||||||||||||||||||||||||||||||||||||||||||||||||
S4 51 GTATGCCAGGGCAGAGAAGAAGGTCAGGGAAGGTCACTGGAGGTCACTGG 100
P4 101 GATACCCTTTCTTCCCACACCANNNGGGAAAGGAGTCCTGCCAGATGACC 150
||||||| |||||||||||| |||||||||||||| |||||||
S4 101 GATACCCNNNCTTCCCACACCAATGGGGAAAGGAGTCCTNNNNGATGACC 150
P4 151 A 151
|
S4 151 A 151