# RImmPort: Quick Start Guide

# Introduction

ImmPort study data is available for download in two formats currently: MySQL and TSV (Tab) formats. The RImmPort workflow is as follows: 1) MySQL formatted study data: User downloads one or more studies in MySQL zip files. Unzips the files. Loads local database instance. Connects to the database. Sets the ImmPort data source to the connection handle. Invokes RImmPort functions. 2) Tab: User downloads one or more studies in Tab format. Passes the folder where the zip files are located to an RImmPort function that builds SQLite database. Connects to the database. Sets the ImmPort data source to the connection handle. Invokes RImmPort functions.

User downloads study data of interest from the ImmPort website ( http://www.immport.org ) **. Depending on the file format MySQL or Tab the data is loaded into a local MySQL and SQLite database respectively. The user installs the RImmPort package, loads the RImmPort library, connects to the ImmPort database, and calls RImmPort methods to load study data from the database into R. Please refer to RImmPort_Article.pdf for a detailed discussion on RImmPort.

** User need to regsiter to the ImmPort website for downloading the datasets.

# Initial Steps

- Download MySQL or Tab formatted data of studies of interest from the ImmPort website
- If working with MySQL-format, load the data in to a local MySQL database
- Install and load RImmPort package, and other required packages.

# Load the RImmPort library

    library(RImmPort)
    library(DBI)
    library(sqldf)
    library(plyr)

# Setup ImmPort data source that all RImmPort functions will use

## Option 1: ImmPort MySQL database

### Download zip files of ImmPort study data in MySQL format. e.g.’SDY139’ and ‘SDY208’

### Load the data into a local MySQL database

### Connect to the ImmPort MySQL database.

    # provide appropriate connection parameters
    mysql_conn <- dbConnect(MySQL(), user="username", password="password",
                       dbname="database",host="host")

### Set the data source as the ImmPort MySQL database.

    setImmPortDataSource(mysql_conn)

## Option 2: ImmPort SQLite database

### Download zip files of ImmPort data, in Tab format. e.g.’SDY139’ and ‘SDY208’

    # get the directory where ImmPort sample data is stored in the directory structure of RImmPort package
    studies_dir <- system.file("extdata", "ImmPortStudies", package = "RImmPort")

    # set tab_dir to the folder where the zip files are located
    tab_dir <- file.path(studies_dir, "Tab")
    list.files(tab_dir)

### Build a local SQLite ImmPort database instance.

    # set db_dir to the folder where the database file 'ImmPort.sqlite' should be stored
    db_dir <- file.path(studies_dir, "Db")

    # build a new ImmPort SQLite database with the data in the downloaded zip files
    buildNewSqliteDb(tab_dir, db_dir)

    list.files(db_dir)

### Connect to the ImmPort SQLite database

    # get the directory of a sample SQLite database that has been bundled into the RImmPort package
    db_dir <- system.file("extdata", "ImmPortStudies", "Db", package = "RImmPort")

    # connect to the private instance of the ImmPort database
    sqlite_conn <- dbConnect(SQLite(), dbname=file.path(db_dir, "ImmPort.sqlite"))

### Set the data source to the ImmPort SQLite DB

    setImmPortDataSource(sqlite_conn)

    ## [1] 1

# NOTE: In rest of the script, all RImmPort functions will use the SQLite ImmPort database as the data source.

# Get all study ids

    getListOfStudies()

    ## [1] "SDY139" "SDY208"

# Get all data of a specific study

The `getStudyFromDatabase` queries the ImmPort database for the entire dataset of a specific study, and instantiates the `Study` reference class with that data.

    ?Study

    # load all the data of study: `SDY139`
    study_id <- 'SDY139'
    sdy139 <- getStudy(study_id)

    ## loading Study ID =  SDY139
    ## loading Demographics data....done
    ## loading Concomitant Medications data....done
    ## loading Exposure data....done
    ## loading Substance Use data....done
    ## loading Adverse Events data....done
    ## loading Protocol Deviations data....done
    ## loading Medical History data....done
    ## loading Associated Persons Medical History data....done
    ## loading Laboratory Test Results data....done
    ## loading Physical Examination data....done
    ## loading Vital Signs data....done
    ## loading Questionnaires data....done
    ## loading Findings About data....done
    ## loading Skin Response data....done
    ## loading Genetics Findings data....loading HLA Typing Results data....done
    ## loading Array Results data....done
    ## done
    ## loading Protein Quantification data....loading ELISA Results data....done
    ## loading MBAA Results data....done
    ## done
    ## loading Cellular Quantification data....loading FCS Results data....done
    ## loading ELISPOT Results data....done
    ## done
    ## loading Nucleic Acid Quantification data....loading PCR Results data....done
    ## done
    ## loading Titer Assay Results data....loading HAI Assay Results data....done
    ## loading Neut. Ab Titer Results data....done
    ## done
    ## loading TrialArms data....done
    ## loading Trial Visits data....done
    ## loading  TrialInclusionExclusionCriteria data....done
    ## loading TrialSummary data.... SDY139  done
    ## done loading Study ID =  SDY139

    # access Demographics data of SDY139
    dm_df <- sdy139$special_purpose$dm_l$dm_df
    head(dm_df)

    ##   STUDYID DOMAIN   USUBJID AGE   AGEU     SEX RACE ETHNIC      SPECIES STRAIN
    ## 1  SDY139     DM SUB118053   2 Months Unknown <NA>   <NA> Mus musculus BALB/c
    ## 2  SDY139     DM SUB118054   2 Months Unknown <NA>   <NA> Mus musculus BALB/c
    ## 3  SDY139     DM SUB118055   2 Months Unknown <NA>   <NA> Mus musculus BALB/c
    ## 4  SDY139     DM SUB118056   2 Months Unknown <NA>   <NA> Mus musculus BALB/c
    ## 5  SDY139     DM SUB118057   2 Months Unknown <NA>   <NA> Mus musculus BALB/c
    ## 6  SDY139     DM SUB118058   2 Months Unknown <NA>   <NA> Mus musculus BALB/c
    ##   SBSTRAIN  ARMCD    ARM
    ## 1     <NA> ARM678 BALB/c
    ## 2     <NA> ARM678 BALB/c
    ## 3     <NA> ARM678 BALB/c
    ## 4     <NA> ARM678 BALB/c
    ## 5     <NA> ARM678 BALB/c
    ## 6     <NA> ARM678 BALB/c

    # access Concomitant Medications data of SDY139
    cm_df <- sdy139$interventions$cm_l$cm_df
    head(cm_df)

    ## NULL

    # get Trial Title from Trial Summary
    ts_df <- sdy139$trial_design$ts_l$ts_df
    title <- ts_df$TSVAL[ts_df$TSPARMCD== "TITLE"]
    title

    ## [1] "The peptide specificity of the endogenous T follicular helper cell repertoire generated after protein immunization"

# Get the list of Domain names.

Note that some RImmPort functions take a domain name as input.

    # get the list of names of all supported Domains
    getListOfDomains()

    ##                           Domain Name Domain Code
    ## 1                      Adverse Events          AE
    ## 2             Concomitant Medications          CM
    ## 3                        Demographics          DM
    ## 4                            Exposure          EX
    ## 5                     Medical History          MH
    ## 6  Associated Persons Medical History        APMH
    ## 7             Laboratory Test Results          LB
    ## 8                Physical Examination          PE
    ## 9                 Protocol Deviations          DV
    ## 10                         Trial Arms          TA
    ## 11                       Trial Visits          TV
    ## 12 Trial Inclusion Exclusion Criteria          TI
    ## 13                      Trial Summary          TS
    ## 14                      Substance Use          SU
    ## 15                        Vital Signs          VS
    ## 16                     Questionnaires          QS
    ## 17                     Findings About          FA
    ## 18                      Skin Response          SR
    ## 19                  Genetics Findings          PF
    ## 20             Protein Quantification          ZA
    ## 21            Cellular Quantification          ZB
    ## 22        Nucleic Acid Quantification          ZC
    ## 23                Titer Assay Results          ZD

    ?"Demographics Domain"

# Get list of studies with specifc domain data

The Domain name should be exact to what is found in the list of Domain names.

    # get list of studies with Cellular Quantification data
    domain_name <- "Cellular Quantification"
    study_ids_l <- getStudiesWithSpecificDomainData(domain_name)
    study_ids_l

    ## [1] "SDY139" "SDY208"

# Get specifc domain data of one or more studies

The Domain name should be exact to what is found in the list of Domain names.

    # get Cellular Quantification data of studies `SDY139` and `SDY208`

    # get domain code of Cellular Quantification domain
    domain_name <- "Cellular Quantification"
    getDomainCode(domain_name)

    ## [1] "ZB"

    study_ids <- c("SDY139", "SDY208")
    domain_name <- "Cellular Quantification"
    zb_l <- getDomainDataOfStudies(domain_name, study_ids)

    ## loading Cellular Quantification data....loading FCS Results data....done
    ## loading ELISPOT Results data....done
    ## done
    ## loading Cellular Quantification data....loading FCS Results data....done
    ## loading ELISPOT Results data....done
    ## done

    if (length(zb_l) > 0)
      names(zb_l)

    ## [1] "zb_df"     "suppzb_df"

    head(zb_l$zb_df)

    ##   STUDYID DOMAIN   USUBJID ZBSEQ       ZBTEST                   ZBCAT
    ## 1  SDY139     ZB SUB118078     1 Figure-7_FCM Cellular Quantification
    ## 2  SDY139     ZB SUB118078     2 Figure-7_FCM Cellular Quantification
    ## 3  SDY139     ZB SUB118078     3 Figure-7_FCM Cellular Quantification
    ## 4  SDY139     ZB SUB118078     4 Figure-7_FCM Cellular Quantification
    ## 5  SDY139     ZB SUB118078     5 Figure-7_FCM Cellular Quantification
    ## 6  SDY139     ZB SUB118078     6 Figure-7_FCM Cellular Quantification
    ##         ZBMETHOD ZBPOPDEF ZBPOPNAM ZBORRES ZBORRESU ZBBASPOP ZBSPEC VISITNUM
    ## 1 Flow Cytometry                                               Cell        1
    ## 2 Flow Cytometry                                               Cell        1
    ## 3 Flow Cytometry                                               Cell        1
    ## 4 Flow Cytometry                                               Cell        1
    ## 5 Flow Cytometry                                               Cell        1
    ## 6 Flow Cytometry                                               Cell        1
    ##                                                      VISIT ZBELTM
    ## 1 Day 0 Protein/peptide inoculation, SL_Sant_Plos1_2012_d0    P0D
    ## 2 Day 0 Protein/peptide inoculation, SL_Sant_Plos1_2012_d0    P0D
    ## 3 Day 0 Protein/peptide inoculation, SL_Sant_Plos1_2012_d0    P0D
    ## 4 Day 0 Protein/peptide inoculation, SL_Sant_Plos1_2012_d0    P0D
    ## 5 Day 0 Protein/peptide inoculation, SL_Sant_Plos1_2012_d0    P0D
    ## 6 Day 0 Protein/peptide inoculation, SL_Sant_Plos1_2012_d0    P0D
    ##                                 ZBTPTREF  ZBREFID                         ZBXFN
    ## 1 Time of initial vaccine administration ES662746   Tfh_Tfh CLN D0-1.297191.fcs
    ## 2 Time of initial vaccine administration ES662746   Tfh_Tfh CLN D0-1.297192.txt
    ## 3 Time of initial vaccine administration ES662766 Tfh 1_Tfh EAR D0-1.297261.fcs
    ## 4 Time of initial vaccine administration ES662766 Tfh 1_Tfh EAR D0-1.297262.txt
    ## 5 Time of initial vaccine administration ES662786   Tfh_Tfh ILN D0-1.297339.fcs
    ## 6 Time of initial vaccine administration ES662786   Tfh_Tfh ILN D0-1.297340.txt

# Get the list of assay types from ImmPort studies

    getListOfAssayTypes()

    ## [1] "ELISA"         "ELISPOT"       "Array"         "PCR"
    ## [5] "HLA Typing"    "MBAA"          "HAI"           "Neut Ab Titer"
    ## [9] "Flow"

# Get specific assay data of one or more Immport studies

The assay type should be exact to what is found in the list of supported assay types.

    # get 'ELISPOT' data of study `SDY139`
    assay_type <- "ELISPOT"
    study_id = "SDY139"
    elispot_l <- getAssayDataOfStudies(study_id, assay_type)

    ## loading Protein Quantification data....done
    ## loading Cellular Quantification data....loading ELISPOT Results data....done
    ## done
    ## loading Nucleic Acid Quantification data....done
    ## loading Titer Assay Results data....done
    ## loading Genetics Findings data....done

    if (length(elispot_l) > 0)
      names(elispot_l)

    ## [1] "zb_df"     "suppzb_df"

    head(elispot_l$zb_df)

    ##   STUDYID DOMAIN   USUBJID ZBSEQ           ZBTEST                   ZBCAT
    ## 1  SDY139     ZB SUB118053  8675 Figure-4_ELISPOT Cellular Quantification
    ## 2  SDY139     ZB SUB118053  8658 Figure-4_ELISPOT Cellular Quantification
    ## 3  SDY139     ZB SUB118053  8673 Figure-4_ELISPOT Cellular Quantification
    ## 4  SDY139     ZB SUB118053  8660 Figure-4_ELISPOT Cellular Quantification
    ## 5  SDY139     ZB SUB118053  8662 Figure-4_ELISPOT Cellular Quantification
    ## 6  SDY139     ZB SUB118053  8663 Figure-4_ELISPOT Cellular Quantification
    ##   ZBMETHOD ZBPOPDEF ZBPOPNAM  ZBORRES ZBORRESU ZBBASPOP ZBSPEC VISITNUM
    ## 1  ELISPOT     IL-2     IL-2 622.8571 1000000             Cell        3
    ## 2  ELISPOT    IL-21    IL-21   8337.5 1000000             Cell        3
    ## 3  ELISPOT     IL-2     IL-2 1048.571 1000000             Cell        3
    ## 4  ELISPOT    IL-21    IL-21   3925.0 1000000             Cell        3
    ## 5  ELISPOT    IL-21    IL-21    600.0 1000000             Cell        3
    ## 6  ELISPOT    IL-21    IL-21 798.5714 1000000             Cell        3
    ##                                            VISIT ZBELTM
    ## 1 Day 8 Sample collection, SL_Sant_Plos1_2012_d8    P8D
    ## 2 Day 8 Sample collection, SL_Sant_Plos1_2012_d8    P8D
    ## 3 Day 8 Sample collection, SL_Sant_Plos1_2012_d8    P8D
    ## 4 Day 8 Sample collection, SL_Sant_Plos1_2012_d8    P8D
    ## 5 Day 8 Sample collection, SL_Sant_Plos1_2012_d8    P8D
    ## 6 Day 8 Sample collection, SL_Sant_Plos1_2012_d8    P8D
    ##                                 ZBTPTREF  ZBREFID ZBXFN
    ## 1 Time of initial vaccine administration ES661770
    ## 2 Time of initial vaccine administration ES661753
    ## 3 Time of initial vaccine administration ES661768
    ## 4 Time of initial vaccine administration ES661755
    ## 5 Time of initial vaccine administration ES661757
    ## 6 Time of initial vaccine administration ES661758

# Serialize RImmPort-formatted study data as .rds files

    # serialize all of the data of studies `SDY139` and `SDY208'
    study_ids <- c('SDY139', 'SDY208')

    # the folder where the .rds files will be stored
    rds_dir <- file.path(studies_dir, "Rds")

    serialzeStudyData(study_ids, rds_dir)
    list.files(rds_dir)

# Load the serialzed data (.rds) files of a specific domain of a study from the directory where the files are located

    # get the directory where ImmPort sample data is stored in the directory structure of RImmPort package
    studies_dir <- system.file("extdata", "ImmPortStudies", package = "RImmPort")

    # the folder where the .rds files will be stored
    rds_dir <- file.path(studies_dir, "Rds")

    # list the studies that have been serialized
    list.files(rds_dir)

    ## [1] "SDY139" "SDY208"

    # load the serialized data of study `SDY208`
    study_id <- 'SDY208'
    dm_l <- loadSerializedStudyData(rds_dir, study_id, "Demographics")

    ##
    ## domain_file_path =  /tmp/RtmpiguLFV/Rinst87d304d220f/RImmPort/extdata/ImmPortStudies/Rds/SDY208/dm.rds
    ## suppdomain_file_path =  /tmp/RtmpiguLFV/Rinst87d304d220f/RImmPort/extdata/ImmPortStudies/Rds/SDY208/suppdm.rds

    head(dm_l[[1]])

    ##   STUDYID DOMAIN   USUBJID AGE  AGEU    SEX RACE ETHNIC      SPECIES STRAIN
    ## 1  SDY208     DM SUB120516   6 Weeks Female <NA>   <NA> Mus musculus   <NA>
    ## 2  SDY208     DM SUB120517   6 Weeks Female <NA>   <NA> Mus musculus   <NA>
    ## 3  SDY208     DM SUB120518   6 Weeks Female <NA>   <NA> Mus musculus   <NA>
    ## 4  SDY208     DM SUB120519   6 Weeks Female <NA>   <NA> Mus musculus   <NA>
    ## 5  SDY208     DM SUB120520   6 Weeks Female <NA>   <NA> Mus musculus   <NA>
    ## 6  SDY208     DM SUB120521   6 Weeks Female <NA>   <NA> Mus musculus   <NA>
    ##   SBSTRAIN  ARMCD
    ## 1     <NA> ARM881
    ## 2     <NA> ARM882
    ## 3     <NA> ARM883
    ## 4     <NA> ARM884
    ## 5     <NA> ARM885
    ## 6     <NA> ARM886
    ##                                                                                                                  ARM
    ## 1                                                 Microneedle vaccination- 5 ug inactivated A/California/04/09 virus
    ## 2                                               Subcutaneous vaccination-  5 ug inactivated A/California/04/09 virus
    ## 3                                                                         Uncoated microneedle vaccination-  Placebo
    ## 4  Microneedle vaccination- 5 ug inactivated A/California/04/09 virus, Challenged: 10x LD50 A/California/04/09 virus
    ## 5 Subcutaneous vaccination- 5 ug inactivated A/California/04/09 virus, Challenged: 10x LD50 A/California/04/09 virus
    ## 6                          Uncoated microneedle vaccination-  Placebo, Challenged: 10x LD50 A/California/04/09 virus