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NestLink an R data package to guide through Engineered Peptide Barcodes for In-Depth Analyzes of Binding Protein Ensembles

Bioconductor version: Release (3.19)

Provides next-generation sequencing (NGS) and mass spectrometry (MS) sample data, code snippets and replication material used for developing NestLink. The NestLink approach is a protein binder selection and identification technology able to biophysically characterize thousands of library members at once without handling individual clones at any stage of the process. Data were acquired on NGS and MS platforms at the Functional Genomics Center Zurich.

Author: Pascal Egloff [aut] , Iwan Zimmermann [ctb] , Fabian M. Arnold [ctb], Cedric A.J. Hutter [ctb] , Lennart Opitz [aut, cre] , Lucy Poveda [ctb] , Hans-Anton Keserue [ctb], Christian Panse [aut, ctb] , Bernd Roschitzki [aut] , Markus Seeger [aut]

Maintainer: Lennart Opitz <lopitz at>

Citation (from within R, enter citation("NestLink")):


To install this package, start R (version "4.4") and enter:

if (!require("BiocManager", quietly = TRUE))


For older versions of R, please refer to the appropriate Bioconductor release.


To view documentation for the version of this package installed in your system, start R and enter:

0. Simulate flycode properties. HTML R Script
1. Link high quality flycode and nanobody sequences by NGS filtering. HTML R Script
2. Analyze flycode detectability using ESP and SSRC prediction HTML R Script
3. Compare Predicted and Observed flycodes hydrophobicity values using F255744. HTML R Script
4. Control experiment to assess the robustness of protein detection via flycodes (NMETH-A35040 Suppl. notes 1). HTML R Script
5. make-data HTML R Script
Reference Manual PDF


biocViews ExperimentData, ExperimentHub, MassSpectrometryData, ReproducibleResearch, SequencingData
Version 1.20.0
License GPL
Depends R (>= 3.6), AnnotationHub(>= 2.15), ExperimentHub(>= 1.0), Biostrings(>= 2.51), gplots (>= 3.0), protViz (>= 0.4), ShortRead(>= 1.41)
Imports grDevices, graphics, stats, utils
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Suggests BiocStyle(>= 2.2), DT, ggplot2, knitr, rmarkdown, testthat, specL, lattice, scales
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Follow Installation instructions to use this package in your R session.

Source Package NestLink_1.20.0.tar.gz
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