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Automatic and interactive quality control for flow cytometry data

Bioconductor version: Release (3.19)

The package is able to perform an automatic or interactive quality control on FCS data acquired using flow cytometry instruments. By evaluating three different properties: 1) flow rate, 2) signal acquisition, 3) dynamic range, the quality control enables the detection and removal of anomalies.

Author: Gianni Monaco, Hao Chen

Maintainer: Gianni Monaco <mongianni1 at>

Citation (from within R, enter citation("flowAI")):


To install this package, start R (version "4.4") and enter:

if (!require("BiocManager", quietly = TRUE))


For older versions of R, please refer to the appropriate Bioconductor release.


To view documentation for the version of this package installed in your system, start R and enter:

Automatic and GUI methods to do quality control on Flow cytometry Data HTML R Script
Reference Manual PDF


biocViews BiomedicalInformatics, FlowCytometry, ImmunoOncology, QualityControl, Software
Version 1.34.0
In Bioconductor since BioC 3.3 (R-3.3) (8 years)
License GPL (>= 2)
Depends R (>= 3.6)
Imports ggplot2, flowCore, plyr, changepoint, knitr, reshape2, RColorBrewer, scales, methods, graphics, stats, utils, rmarkdown
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Follow Installation instructions to use this package in your R session.

Source Package flowAI_1.34.0.tar.gz
Windows Binary (64-bit only)
macOS Binary (x86_64) flowAI_1.34.0.tgz
macOS Binary (arm64) flowAI_1.34.0.tgz
Source Repository git clone
Source Repository (Developer Access) git clone
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