Contents

1 Summary of the output objects of scTensor

Here, we introduced the objects saved in reanalysis.RData.

library("scTensor")
load("reanalysis.RData")

After performing cellCellReport, some R objects are saved in the reanalysis.RData as follows;

2 Execution of scTensor with the different options

Using the reanalysis.RData, some users may want to perform scTensor with different parameters.

For example, some users want to perform cellCellDecomp with different ranks, perform cellCellReport with omitting some enrichment analysis, provide the results to their collaborators.

To do such tasks, just type like belows.

library("AnnotationHub")
library("LRBaseDbi")

# Create LRBase object
ah <- AnnotationHub()
dbfile <- query(ah, c("LRBaseDb", "Homo sapiens", "v002"))[[1]]
LRBase.Hsa.eg.db <- LRBaseDbi::LRBaseDb(dbfile)

# Register the file pass of user's LRBase
metadata(sce)$lrbase <- dbfile(LRBase.Hsa.eg.db)

# CCI Tensor Decomposition
cellCellDecomp(sce, ranks=c(6,5), assayNames="normcounts")

# HTML Report
cellCellReport(sce, reducedDimNames="TSNE", assayNames="normcounts",
    title="Cell-cell interaction within Germline_Male, GSE86146",
    author="Koki Tsuyuzaki", html.open=TRUE,
    goenrich=TRUE, meshenrich=FALSE, reactomeenrich=FALSE,
    doenrich=FALSE, ncgenrich=FALSE, dgnenrich=FALSE)

Session information

## R version 4.3.0 RC (2023-04-13 r84269)
## Platform: x86_64-pc-linux-gnu (64-bit)
## Running under: Ubuntu 22.04.2 LTS
## 
## Matrix products: default
## BLAS:   /home/biocbuild/bbs-3.17-bioc/R/lib/libRblas.so 
## LAPACK: /usr/lib/x86_64-linux-gnu/lapack/liblapack.so.3.10.0
## 
## locale:
##  [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C              
##  [3] LC_TIME=en_GB              LC_COLLATE=C              
##  [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8   
##  [7] LC_PAPER=en_US.UTF-8       LC_NAME=C                 
##  [9] LC_ADDRESS=C               LC_TELEPHONE=C            
## [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C       
## 
## time zone: America/New_York
## tzcode source: system (glibc)
## 
## attached base packages:
## [1] stats4    stats     graphics  grDevices utils     datasets  methods  
## [8] base     
## 
## other attached packages:
##  [1] scTGIF_1.14.0                          
##  [2] Homo.sapiens_1.3.1                     
##  [3] TxDb.Hsapiens.UCSC.hg19.knownGene_3.2.2
##  [4] org.Hs.eg.db_3.17.0                    
##  [5] GO.db_3.17.0                           
##  [6] OrganismDbi_1.42.0                     
##  [7] GenomicFeatures_1.52.0                 
##  [8] AnnotationDbi_1.62.0                   
##  [9] SingleCellExperiment_1.22.0            
## [10] SummarizedExperiment_1.30.0            
## [11] Biobase_2.60.0                         
## [12] GenomicRanges_1.52.0                   
## [13] GenomeInfoDb_1.36.0                    
## [14] IRanges_2.34.0                         
## [15] S4Vectors_0.38.0                       
## [16] MatrixGenerics_1.12.0                  
## [17] matrixStats_0.63.0                     
## [18] scTensor_2.10.0                        
## [19] RSQLite_2.3.1                          
## [20] LRBaseDbi_2.10.0                       
## [21] AnnotationHub_3.8.0                    
## [22] BiocFileCache_2.8.0                    
## [23] dbplyr_2.3.2                           
## [24] BiocGenerics_0.46.0                    
## [25] BiocStyle_2.28.0                       
## 
## loaded via a namespace (and not attached):
##   [1] rTensor_1.4.8                 GSEABase_1.62.0              
##   [3] progress_1.2.2                goftest_1.2-3                
##   [5] Biostrings_2.68.0             vctrs_0.6.2                  
##   [7] spatstat.random_3.1-4         digest_0.6.31                
##   [9] png_0.1-8                     registry_0.5-1               
##  [11] ggrepel_0.9.3                 deldir_1.0-6                 
##  [13] parallelly_1.35.0             magick_2.7.4                 
##  [15] MASS_7.3-59                   reshape2_1.4.4               
##  [17] httpuv_1.6.9                  foreach_1.5.2                
##  [19] qvalue_2.32.0                 withr_2.5.0                  
##  [21] xfun_0.39                     ggfun_0.0.9                  
##  [23] ellipsis_0.3.2                survival_3.5-5               
##  [25] memoise_2.0.1                 hexbin_1.28.3                
##  [27] gson_0.1.0                    tidytree_0.4.2               
##  [29] zoo_1.8-12                    pbapply_1.7-0                
##  [31] entropy_1.3.1                 prettyunits_1.1.1            
##  [33] KEGGREST_1.40.0               promises_1.2.0.1             
##  [35] httr_1.4.5                    restfulr_0.0.15              
##  [37] schex_1.14.0                  globals_0.16.2               
##  [39] fitdistrplus_1.1-11           miniUI_0.1.1.1               
##  [41] generics_0.1.3                DOSE_3.26.0                  
##  [43] reactome.db_1.84.0            babelgene_22.9               
##  [45] concaveman_1.1.0              curl_5.0.0                   
##  [47] fields_14.1                   zlibbioc_1.46.0              
##  [49] ggraph_2.1.0                  polyclip_1.10-4              
##  [51] ca_0.71.1                     GenomeInfoDbData_1.2.10      
##  [53] RBGL_1.76.0                   interactiveDisplayBase_1.38.0
##  [55] xtable_1.8-4                  stringr_1.5.0                
##  [57] evaluate_0.20                 hms_1.1.3                    
##  [59] bookdown_0.33                 irlba_2.3.5.1                
##  [61] colorspace_2.1-0              filelock_1.0.2               
##  [63] visNetwork_2.1.2              ROCR_1.0-11                  
##  [65] reticulate_1.28               spatstat.data_3.0-1          
##  [67] magrittr_2.0.3                lmtest_0.9-40                
##  [69] Rgraphviz_2.44.0              later_1.3.0                  
##  [71] viridis_0.6.2                 ggtree_3.8.0                 
##  [73] lattice_0.21-8                misc3d_0.9-1                 
##  [75] spatstat.geom_3.1-0           future.apply_1.10.0          
##  [77] genefilter_1.82.0             plot3D_1.4                   
##  [79] scattermore_0.8               XML_3.99-0.14                
##  [81] shadowtext_0.1.2              cowplot_1.1.1                
##  [83] RcppAnnoy_0.0.20              pillar_1.9.0                 
##  [85] nlme_3.1-162                  iterators_1.0.14             
##  [87] compiler_4.3.0                stringi_1.7.12               
##  [89] Category_2.66.0               TSP_1.2-4                    
##  [91] tensor_1.5                    dendextend_1.17.1            
##  [93] GenomicAlignments_1.36.0      plyr_1.8.8                   
##  [95] msigdbr_7.5.1                 BiocIO_1.10.0                
##  [97] crayon_1.5.2                  abind_1.4-5                  
##  [99] gridGraphics_0.5-1            sp_1.6-0                     
## [101] graphlayouts_0.8.4            bit_4.0.5                    
## [103] dplyr_1.1.2                   fastmatch_1.1-3              
## [105] tagcloud_0.6                  codetools_0.2-19             
## [107] bslib_0.4.2                   plotly_4.10.1                
## [109] mime_0.12                     splines_4.3.0                
## [111] Rcpp_1.0.10                   HDO.db_0.99.1                
## [113] knitr_1.42                    blob_1.2.4                   
## [115] utf8_1.2.3                    BiocVersion_3.17.1           
## [117] listenv_0.9.0                 checkmate_2.1.0              
## [119] ggplotify_0.1.0               tibble_3.2.1                 
## [121] Matrix_1.5-4                  tweenr_2.0.2                 
## [123] pkgconfig_2.0.3               tools_4.3.0                  
## [125] cachem_1.0.7                  viridisLite_0.4.1            
## [127] DBI_1.1.3                     graphite_1.46.0              
## [129] fastmap_1.1.1                 rmarkdown_2.21               
## [131] scales_1.2.1                  grid_4.3.0                   
## [133] outliers_0.15                 ica_1.0-3                    
## [135] Seurat_4.3.0                  Rsamtools_2.16.0             
## [137] sass_0.4.5                    patchwork_1.1.2              
## [139] BiocManager_1.30.20           dotCall64_1.0-2              
## [141] graph_1.78.0                  RANN_2.6.1                   
## [143] farver_2.1.1                  tidygraph_1.2.3              
## [145] scatterpie_0.1.9              yaml_2.3.7                   
## [147] AnnotationForge_1.42.0        rtracklayer_1.60.0           
## [149] cli_3.6.1                     purrr_1.0.1                  
## [151] webshot_0.5.4                 leiden_0.4.3                 
## [153] lifecycle_1.0.3               uwot_0.1.14                  
## [155] backports_1.4.1               BiocParallel_1.34.0          
## [157] annotate_1.78.0               MeSHDbi_1.36.0               
## [159] rjson_0.2.21                  gtable_0.3.3                 
## [161] ggridges_0.5.4                progressr_0.13.0             
## [163] parallel_4.3.0                ape_5.7-1                    
## [165] jsonlite_1.8.4                seriation_1.4.2              
## [167] bitops_1.0-7                  ggplot2_3.4.2                
## [169] bit64_4.0.5                   assertthat_0.2.1             
## [171] Rtsne_0.16                    yulab.utils_0.0.6            
## [173] ReactomePA_1.44.0             spatstat.utils_3.0-2         
## [175] SeuratObject_4.1.3            heatmaply_1.4.2              
## [177] jquerylib_0.1.4               highr_0.10                   
## [179] nnTensor_1.1.13               GOSemSim_2.26.0              
## [181] ccTensor_1.0.2                lazyeval_0.2.2               
## [183] shiny_1.7.4                   htmltools_0.5.5              
## [185] enrichplot_1.20.0             sctransform_0.3.5            
## [187] rappdirs_0.3.3                glue_1.6.2                   
## [189] tcltk_4.3.0                   spam_2.9-1                   
## [191] XVector_0.40.0                RCurl_1.98-1.12              
## [193] treeio_1.24.0                 gridExtra_2.3                
## [195] igraph_1.4.2                  R6_2.5.1                     
## [197] tidyr_1.3.0                   fdrtool_1.2.17               
## [199] cluster_2.1.4                 aplot_0.1.10                 
## [201] DelayedArray_0.26.0           tidyselect_1.2.0             
## [203] plotrix_3.8-2                 GOstats_2.66.0               
## [205] maps_3.4.1                    xml2_1.3.3                   
## [207] ggforce_0.4.1                 future_1.32.0                
## [209] munsell_0.5.0                 KernSmooth_2.23-20           
## [211] data.table_1.14.8             htmlwidgets_1.6.2            
## [213] fgsea_1.26.0                  RColorBrewer_1.1-3           
## [215] biomaRt_2.56.0                rlang_1.1.0                  
## [217] spatstat.sparse_3.0-1         meshr_2.6.0                  
## [219] spatstat.explore_3.1-0        fansi_1.0.4