Functions for normalisation of RT-qPCR data


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Documentation for package ‘NormqPCR’ version 1.46.0

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NormqPCR-package Functions for normalisation of RT-qPCR data.
Bladder Bladder dataset of Andersen et al (2004)
BladderRepro Dataset of Andersen et al (2004)
BladderRepro.qPCRBatch Dataset of Andersen et al (2004)
Colon Colon dataset of Andersen et al (2004)
combineTechReps Combines Technical Replicates
combineTechReps-method Combines Technical Replicates
combineTechRepsWithSD Combines Technical Replicates
combineTechRepsWithSD-method Combines Technical Replicates
ComputeNRQs Compute Normalized Relative Quantities
ComputeNRQs-method Compute Normalized Relative Quantities
ComputeNRQs-methods Compute Normalized Relative Quantities
CqValues Compute Cq value and amplification efficiency
CqValues-method Compute Cq value and amplification efficiency
CqValues-methods Compute Cq value and amplification efficiency
deltaCq Perform normalization with a given housekeeping gene
deltaCt Perform normalization with a given housekeeping gene
deltaCt-method Perform normalization with a given housekeeping gene
deltaCt-methods Perform normalization with a given housekeeping gene
deltaDeltaCq Perform normalization and differential expression with given housekeeping gene
deltaDeltaCq-method Perform normalization and differential expression with given housekeeping gene
deltaDeltaCq-methods Perform normalization and differential expression with given housekeeping gene
deltaDeltaCt Perform normalization and differential expression with given housekeeping gene
deltaDeltaCt-method Perform normalization and differential expression with given housekeeping gene
deltaDeltaCt-methods Perform normalization and differential expression with given housekeeping gene
geNorm Data set of Vandesompele et al (2002)
geNorm.qPCRBatch Data set of Vandesompele et al (2002)
geomMean Geometric Mean
makeAllNAs Make all Cq values NA
makeAllNAs-method Make all Cq values NA
makeAllNewVal Make all Cq values NA
makeAllNewVal-method Make all Cq values NA
NormqPCR Functions for normalisation of RT-qPCR data.
replaceAboveCutOff Replace Cq values with new value
replaceAboveCutOff-method Replace Cq values with new value
replaceNAs Replace NAs with a given value
replaceNAs-method Replace NAs with a given value
selectHKs Selection of reference/housekeeping genes
selectHKs-method Selection of reference/housekeeping genes
selectHKs-methods Selection of reference/housekeeping genes
stabMeasureM Gene expression stability value M
stabMeasureRho Gene expression stability value rho
stabMeasureRho-method Gene expression stability value rho
stabMeasureRho-methods Gene expression stability value rho