Compiled date: 2022-11-01

Last edited: 2020-04-20

License: MIT + file LICENSE

1 Background

Users can define their own custom plots or tables to include in the iSEE interface (Rue-Albrecht et al. 2018). These custom panels are intended to receive subsets of rows and/or columns from other transmitting panels in the interface. The values in the custom panels are then recomputed on the fly by user-defined functions using the transmitted subset. This provides a flexible and convenient framework for lightweight interactive analysis during data exploration. For example, selection of a particular subset of samples can be transmitted to a custom plot panel that performs dimensionality reduction on that subset. Alternatively, the subset can be transmitted to a custom table that performs a differential expression analysis between that subset and all other samples.

2 Defining custom functions

2.1 Minimum requirements

Recalculations in custom panels are performed using user-defined functions that are supplied to the iSEE() call. The only requirements are that the function must accept:

  • A SummarizedExperiment object or its derivatives as the first argument.
  • A list of character vectors containing row names as the second argument. Each vector specifies the rows that are currently selected in the transmitting row-based panel, as an active selection or as one of the saved selection. This may be NULL if no transmitting panel was selected or if no selections are available.
  • A list of character vectors containing column names as the third argument. Each vector specifies the columns that are currently selected in the transmitting column-based panel, as an active selection or as one of the saved selection. This may be NULL if no transmitting panel was selected or if no selections are available.

The output of the function should be:

  • A ggplot object for functions used in custom plot panels.
  • A data.frame for functions used in custom table panels.

2.2 Example of custom plot panel

To demonstrate the use of custom plot panels, we define an example function CUSTOM_DIMRED that takes a subset of features and cells in a SingleCellExperiment object and performs dimensionality reduction on that subset with scater function (McCarthy et al. 2017).

library(scater)
CUSTOM_DIMRED <- function(se, rows, columns, ntop=500, scale=TRUE,
    mode=c("PCA", "TSNE", "UMAP"))
{
    print(columns)
    if (is.null(columns)) {
        return(
            ggplot() + theme_void() + geom_text(
                aes(x, y, label=label),
                data.frame(x=0, y=0, label="No column data selected."),
                size=5)
            )
    }

    mode <- match.arg(mode)
    if (mode=="PCA") {
        calcFUN <- runPCA
    } else if (mode=="TSNE") {
        calcFUN <- runTSNE
    } else if (mode=="UMAP") {
        calcFUN <- runUMAP
    }

    set.seed(1000)
    kept <- se[, unique(unlist(columns))]
    kept <- calcFUN(kept, ncomponents=2, ntop=ntop,
        scale=scale, subset_row=unique(unlist(rows)))
    plotReducedDim(kept, mode)
}

As mentioned above, rows and columns may be NULL if no selection was made in the respective transmitting panels. How these should be treated is up to the user-defined function. In this example, an empty ggplot is returned if there is no selection on the columns, while the default behaviour of runPCA, runTSNE, etc. is used if rows=NULL.

To create instances of our panel, we call the createCustomPlot() function with CUSTOM_DIMRED to set up the custom plot class and its methods. This returns a constructor function that can be directly used to generate an instance of our custom plot.

library(iSEE)
GENERATOR <- createCustomPlot(CUSTOM_DIMRED)
custom_panel <- GENERATOR()
class(custom_panel)
#> [1] "CustomPlot"
#> attr(,"package")
#> [1] ".GlobalEnv"

We can now easily supply instances of our new custom plot class to iSEE() like any other Panel instance. The example below creates an application where a column data plot transmits a selection to our custom plot, the latter of which is initialized in \(t\)-SNE mode with the top 1000 most variable genes.

# NOTE: as mentioned before, you don't have to create 'BrushData' manually;
# just open an app, make a brush and copy it from the panel settings.
cdp <- ColumnDataPlot(
    XAxis="Column data", 
    XAxisColumnData="Primary.Type", 
    PanelId=1L,
    BrushData=list(
        xmin = 10.1, xmax = 15.0, ymin = 5106720.6, ymax = 28600906.0, 
        coords_css = list(xmin = 271.0, xmax = 380.0, ymin = 143.0, ymax = 363.0), 
        coords_img = list(xmin = 352.3, xmax = 494.0, ymin = 185.9, ymax = 471.9), 
        img_css_ratio = list(x = 1.3, y = 1.2), 
        mapping = list(x = "X", y = "Y", group = "GroupBy"),
        domain = list(
            left = 0.4, right = 17.6, bottom = -569772L, top = 41149532L, 
            discrete_limits = list(
                x = list("L4 Arf5", "L4 Ctxn3", "L4 Scnn1a", 
                    "L5 Ucma", "L5a Batf3", "L5a Hsd11b1", "L5a Pde1c", 
                    "L5a Tcerg1l", "L5b Cdh13", "L5b Chrna6", "L5b Tph2", 
                    "L6a Car12", "L6a Mgp", "L6a Sla", "L6a Syt17", 
                    "Pvalb Tacr3", "Sst Myh8")
            )
        ), 
        range = list(
            left = 68.986301369863, right = 566.922374429224, 
            bottom = 541.013698630137, top = 33.1552511415525
        ), 
        log = list(x = NULL, y = NULL), 
        direction = "xy", 
        brushId = "ColumnDataPlot1_Brush", 
        outputId = "ColumnDataPlot1"
    )
)

custom.p <- GENERATOR(mode="TSNE", ntop=1000, 
    ColumnSelectionSource="ColumnDataPlot1")

app <- iSEE(sce, initial=list(cdp, custom.p))

The most interesting aspect of createCustomPlot() is that the UI elements for modifying the optional arguments in CUSTOM_DIMRED are also automatically generated. This provides a convenient way to generate a reasonably intuitive UI for rapid prototyping, though there are limitations - see the documentation for more details.

2.3 Example of custom table panel

To demonstrate the use of custom table panels, we define an example function CUSTOM_SUMMARY below. This takes a subset of features and cells in a SingleCellExperiment object and creates dataframe that details the mean, variance and count of samples with expression above a given cut-off within the selection. If either rows or columns are NULL, all rows or columns are used, respectively.

CUSTOM_SUMMARY <- function(se, ri, ci, assay="logcounts", min_exprs=0) {
    if (is.null(ri)) {
        ri <- rownames(se)
    } else {
        ri <- unique(unlist(ri))
    }
    if (is.null(ci)) {
        ci <- colnames(se)
    } else {
        ci <- unique(unlist(ci))
    }
    
    assayMatrix <- assay(se, assay)[ri, ci, drop=FALSE]
    
    data.frame(
        Mean = rowMeans(assayMatrix),
        Var = rowVars(assayMatrix),
        Sum = rowSums(assayMatrix),
        n_detected = rowSums(assayMatrix > min_exprs),
        row.names = ri
    )
}

To create instances of our panel, we call the createCustomTable() function with CUSTOM_SUMMARY, which again returns a constructor function that can be used directly in iSEE(). Again, the function will attempt to auto-pick an appropriate UI element for each optional argument in CUSTOM_SUMMARY.

library(iSEE)
GENERATOR <- createCustomTable(CUSTOM_SUMMARY)
custom.t <- GENERATOR(PanelWidth=8L,
    ColumnSelectionSource="ReducedDimensionPlot1",
    SearchColumns=c("", "17.8 ... 10000", "", "") # filtering for HVGs.
) 
class(custom.t)
#> [1] "CustomTable"
#> attr(,"package")
#> [1] ".GlobalEnv"

# Preselecting some points in the reduced dimension plot.
# Again, you don't have to manually create the 'BrushData'!
rdp <- ReducedDimensionPlot(
    PanelId=1L,
    BrushData = list(
        xmin = -44.8, xmax = -14.3, ymin = 7.5, ymax = 47.1, 
        coords_css = list(xmin = 55.0, xmax = 169.0, ymin = 48.0, ymax = 188.0),
        coords_img = list(xmin = 71.5, xmax = 219.7, ymin = 62.4, ymax = 244.4), 
        img_css_ratio = list(x = 1.3, y = 1.29), 
        mapping = list(x = "X", y = "Y"), 
        domain = list(left = -49.1, right = 57.2, bottom = -70.3, top = 53.5), 
        range = list(left = 50.9, right = 566.9, bottom = 603.0, top = 33.1), 
        log = list(x = NULL, y = NULL), 
        direction = "xy", 
        brushId = "ReducedDimensionPlot1_Brush", 
        outputId = "ReducedDimensionPlot1"
    )
)

app <- iSEE(sce, initial=list(rdp, custom.t))

3 Handling active and saved selections

Recall that the second and third arguments are actually lists containing both active and saved selections from the transmitter. More advanced custom panels can take advantage of these multiple selections to perform more sophisticated data processing. For example, we can write a function that computes log-fold changes between the samples in the active selection and the samples in each saved selection. (It would be trivial to extend this to obtain actual differential expression statistics, e.g., using scran::findMarkers() or functions from packages like limma.)

CUSTOM_DIFFEXP <- function(se, ri, ci, assay="logcounts") {
    ri <- ri$active
    if (is.null(ri)) { 
        ri <- rownames(se)
    }
    assayMatrix <- assay(se, assay)[ri, , drop=FALSE]

    if (is.null(ci$active) || length(ci)<2L) {
        return(data.frame(row.names=character(0), LogFC=integer(0))) # dummy value.
    }
    active <- rowMeans(assayMatrix[,ci$active,drop=FALSE])

    all_saved <- ci[grep("saved", names(ci))]
    lfcs <- vector("list", length(all_saved))
    for (i in seq_along(lfcs)) {
        saved <- rowMeans(assayMatrix[,all_saved[[i]],drop=FALSE])
        lfcs[[i]] <- active - saved
    }

    names(lfcs) <- sprintf("LogFC/%i", seq_along(lfcs))
    do.call(data.frame, lfcs)
}

We also re-use these statistics to visualize some of the genes with the largest log-fold changes:

CUSTOM_HEAT <- function(se, ri, ci, assay="logcounts") {
    everything <- CUSTOM_DIFFEXP(se, ri, ci, assay=assay)
    if (nrow(everything) == 0L) {
        return(ggplot()) # empty ggplot if no genes reported.
    }

    everything <- as.matrix(everything)
    top <- head(order(rowMeans(abs(everything)), decreasing=TRUE), 50)
    topFC <- everything[top, , drop=FALSE]
    dfFC <- data.frame(
        gene=rep(rownames(topFC), ncol(topFC)),
        contrast=rep(colnames(topFC), each=nrow(topFC)),
        value=as.vector(topFC)
    )
    ggplot(dfFC, aes(contrast, gene)) + geom_raster(aes(fill = value))
}

We test this out as shown below. Note that each saved selection is also the active selection when it is first generated, hence the log-fold changes of zero in the last column of the heat map until a new active selection is drawn.

TAB_GEN <- createCustomTable(CUSTOM_DIFFEXP)
HEAT_GEN <- createCustomPlot(CUSTOM_HEAT)

rdp[["SelectionHistory"]] <- list(
    list(lasso = NULL, closed = TRUE, panelvar1 = NULL, panelvar2 = NULL, 
        mapping = list(x = "X", y = "Y"), 
        coord = structure(c(-44.3, -23.7, -13.5, -19.6,
            -33.8, -48.6, -44.3, -33.9, -55.4, -43.0,
            -19.5, -4.0, -22.6, -33.9), .Dim = c(7L, 2L)
        )
    )
)

app <- iSEE(sce, initial=list(rdp,
    TAB_GEN(ColumnSelectionSource="ReducedDimensionPlot1"),
    HEAT_GEN(ColumnSelectionSource="ReducedDimensionPlot1"))
)

4 Advanced extensions

The system described above is rather limited and is only provided for quick-and-dirty customizations. For more serious extensions, we provide a S4 framework for native integration of user-created panels into the application. This allows specification of custom interface elements and observers and transmission of multiple selections to other panels. Prospective panel developers are advised to read the book, as there are too many cool things that will not fit into this vignette.

Session Info

sessionInfo()
#> R version 4.2.1 (2022-06-23)
#> Platform: x86_64-pc-linux-gnu (64-bit)
#> Running under: Ubuntu 20.04.5 LTS
#> 
#> Matrix products: default
#> BLAS:   /home/biocbuild/bbs-3.16-bioc/R/lib/libRblas.so
#> LAPACK: /home/biocbuild/bbs-3.16-bioc/R/lib/libRlapack.so
#> 
#> locale:
#>  [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C              
#>  [3] LC_TIME=en_GB              LC_COLLATE=C              
#>  [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8   
#>  [7] LC_PAPER=en_US.UTF-8       LC_NAME=C                 
#>  [9] LC_ADDRESS=C               LC_TELEPHONE=C            
#> [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C       
#> 
#> attached base packages:
#> [1] stats4    stats     graphics  grDevices utils     datasets  methods  
#> [8] base     
#> 
#> other attached packages:
#>  [1] TENxPBMCData_1.15.0         HDF5Array_1.26.0           
#>  [3] rhdf5_2.42.0                DelayedArray_0.24.0        
#>  [5] Matrix_1.5-1                scater_1.26.0              
#>  [7] ggplot2_3.3.6               scuttle_1.8.0              
#>  [9] scRNAseq_2.11.0             iSEE_2.10.0                
#> [11] SingleCellExperiment_1.20.0 SummarizedExperiment_1.28.0
#> [13] Biobase_2.58.0              GenomicRanges_1.50.0       
#> [15] GenomeInfoDb_1.34.0         IRanges_2.32.0             
#> [17] S4Vectors_0.36.0            BiocGenerics_0.44.0        
#> [19] MatrixGenerics_1.10.0       matrixStats_0.62.0         
#> [21] BiocStyle_2.26.0           
#> 
#> loaded via a namespace (and not attached):
#>   [1] circlize_0.4.15               AnnotationHub_3.6.0          
#>   [3] BiocFileCache_2.6.0           igraph_1.3.5                 
#>   [5] lazyeval_0.2.2                shinydashboard_0.7.2         
#>   [7] splines_4.2.1                 BiocParallel_1.32.0          
#>   [9] digest_0.6.30                 ensembldb_2.22.0             
#>  [11] foreach_1.5.2                 htmltools_0.5.3              
#>  [13] viridis_0.6.2                 fansi_1.0.3                  
#>  [15] magrittr_2.0.3                memoise_2.0.1                
#>  [17] ScaledMatrix_1.6.0            cluster_2.1.4                
#>  [19] doParallel_1.0.17             ComplexHeatmap_2.14.0        
#>  [21] Biostrings_2.66.0             prettyunits_1.1.1            
#>  [23] colorspace_2.0-3              blob_1.2.3                   
#>  [25] rappdirs_0.3.3                ggrepel_0.9.1                
#>  [27] xfun_0.34                     dplyr_1.0.10                 
#>  [29] crayon_1.5.2                  RCurl_1.98-1.9               
#>  [31] jsonlite_1.8.3                iterators_1.0.14             
#>  [33] glue_1.6.2                    gtable_0.3.1                 
#>  [35] zlibbioc_1.44.0               XVector_0.38.0               
#>  [37] GetoptLong_1.0.5              BiocSingular_1.14.0          
#>  [39] Rhdf5lib_1.20.0               shape_1.4.6                  
#>  [41] scales_1.2.1                  DBI_1.1.3                    
#>  [43] miniUI_0.1.1.1                Rcpp_1.0.9                   
#>  [45] viridisLite_0.4.1             xtable_1.8-4                 
#>  [47] progress_1.2.2                clue_0.3-62                  
#>  [49] rsvd_1.0.5                    bit_4.0.4                    
#>  [51] DT_0.26                       htmlwidgets_1.5.4            
#>  [53] httr_1.4.4                    RColorBrewer_1.1-3           
#>  [55] shinyAce_0.4.2                ellipsis_0.3.2               
#>  [57] pkgconfig_2.0.3               XML_3.99-0.12                
#>  [59] sass_0.4.2                    dbplyr_2.2.1                 
#>  [61] utf8_1.2.2                    tidyselect_1.2.0             
#>  [63] rlang_1.0.6                   later_1.3.0                  
#>  [65] AnnotationDbi_1.60.0          munsell_0.5.0                
#>  [67] BiocVersion_3.16.0            tools_4.2.1                  
#>  [69] cachem_1.0.6                  cli_3.4.1                    
#>  [71] generics_0.1.3                RSQLite_2.2.18               
#>  [73] ExperimentHub_2.6.0           rintrojs_0.3.2               
#>  [75] evaluate_0.17                 stringr_1.4.1                
#>  [77] fastmap_1.1.0                 yaml_2.3.6                   
#>  [79] knitr_1.40                    bit64_4.0.5                  
#>  [81] purrr_0.3.5                   AnnotationFilter_1.22.0      
#>  [83] KEGGREST_1.38.0               sparseMatrixStats_1.10.0     
#>  [85] nlme_3.1-160                  mime_0.12                    
#>  [87] xml2_1.3.3                    biomaRt_2.54.0               
#>  [89] compiler_4.2.1                beeswarm_0.4.0               
#>  [91] filelock_1.0.2                curl_4.3.3                   
#>  [93] png_0.1-7                     interactiveDisplayBase_1.36.0
#>  [95] tibble_3.1.8                  bslib_0.4.0                  
#>  [97] stringi_1.7.8                 highr_0.9                    
#>  [99] GenomicFeatures_1.50.0        lattice_0.20-45              
#> [101] ProtGenerics_1.30.0           shinyjs_2.1.0                
#> [103] vctrs_0.5.0                   rhdf5filters_1.10.0          
#> [105] pillar_1.8.1                  lifecycle_1.0.3              
#> [107] BiocManager_1.30.19           jquerylib_0.1.4              
#> [109] GlobalOptions_0.1.2           BiocNeighbors_1.16.0         
#> [111] irlba_2.3.5.1                 bitops_1.0-7                 
#> [113] rtracklayer_1.58.0            httpuv_1.6.6                 
#> [115] R6_2.5.1                      BiocIO_1.8.0                 
#> [117] bookdown_0.29                 promises_1.2.0.1             
#> [119] gridExtra_2.3                 vipor_0.4.5                  
#> [121] codetools_0.2-18              colourpicker_1.2.0           
#> [123] assertthat_0.2.1              fontawesome_0.4.0            
#> [125] rjson_0.2.21                  withr_2.5.0                  
#> [127] shinyWidgets_0.7.4            GenomicAlignments_1.34.0     
#> [129] Rsamtools_2.14.0              GenomeInfoDbData_1.2.9       
#> [131] mgcv_1.8-41                   parallel_4.2.1               
#> [133] hms_1.1.2                     beachmat_2.14.0              
#> [135] grid_4.2.1                    DelayedMatrixStats_1.20.0    
#> [137] rmarkdown_2.17                Rtsne_0.16                   
#> [139] shiny_1.7.3                   ggbeeswarm_0.6.0             
#> [141] restfulr_0.0.15
# devtools::session_info()

References

McCarthy, D. J., K. R. Campbell, A. T. Lun, and Q. F. Wills. 2017. “Scater: pre-processing, quality control, normalization and visualization of single-cell RNA-seq data in R.” Bioinformatics 33 (8): 1179–86.

Rue-Albrecht, K., F. Marini, C. Soneson, and A. T. L. Lun. 2018. “ISEE: Interactive Summarizedexperiment Explorer.” F1000Research 7 (June): 741.