zFactor {synlet} | R Documentation |
calcualte the Z and Z' factor for each plate.
zFactor(dat, negativeCon, positiveCon, useMean = TRUE)
dat |
synthetic lethal RNAi screen data. |
negativeCon |
the negative control used in the WELL_CONTENT_NAME. |
positiveCon |
the positive control used in the WELL_CONTENT_NAME. |
useMean |
use mean to calcualate z factor and z' factor by default; otherwise use median. |
A data.frame contains z factor and z' factor
Zhang J.H., Chung T.D. & Oldenburg K.R. A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J. Biomol. Screen. B, 4 67-73 (1999). Birmingham,A. et al. (2009) Statistical methods for analysis of high-throughput RNA interference screens. Nat Methods, 6, 569-575.
zFactor(exampleDat, negativeCon = "scrambled control si1", positiveCon = "PLK1 si1")