extend_pe_to_gg {multicrispr} | R Documentation |
Extend prime editing target to find GG sites in accessible neighbourhood
extend_pe_to_gg(gr, nrt = 16, plot = FALSE)
gr |
target |
nrt |
n RT nucleotides (default 16, recommended 10-16) |
plot |
TRUE or FALSE (default) |
Extends each target range to the area in which to search for a prime editing GG duplet, as shown in the sketch below.
===============> —-GG———> —-GG———> ** <———GG— <———GG—- <===============
# PE example #----------- require(magrittr) bsgenome <- BSgenome.Hsapiens.UCSC.hg38::BSgenome.Hsapiens.UCSC.hg38 gr <- char_to_granges(c(PRNP = 'chr20:4699600:+', # snp HBB = 'chr11:5227002:-', # snp HEXA = 'chr15:72346580-72346583:-', # del CFTR = 'chr7:117559593-117559595:+'), # ins bsgenome) extend_pe_to_gg(gr, plot = TRUE)