specUnmix {flowSpecs} | R Documentation |
This function performs the central task of spectral unmixing, to convert the raw photon detector input to "biological" proxy-signals.
specUnmix(flowObj, specMat)
flowObj |
The fcs object to be filtered. Both flowFrames and flowSets are accepted. |
specMat |
This is a matrix generated by the secMatCalc function, possibly with edited row names. |
The unmixed data. It will be returned in the format it was imported as.
# Load uncompensated data data(fullPanel) # Load the spectral unmixing matrix generated with controls from the same # experiment. These can be generated using the specMatCalc function. data(specMat) # And now, just run the function fullPanelUnmix <- specUnmix(fullPanel, specMat)