newFlowframe {cyanoFilter} | R Documentation |
takes a flowframe, a group indicator and formulates another flowframe with group indicator as part of the expression matrix of the new flowframe.
newFlowframe(flowfile, group = NULL, togate = NULL)
flowfile |
flowframe after debris are removed. |
group |
cluster group to be added to the expression matrix |
togate |
channel detected to have more than one peak |
flowframe with indicators for particle cluster
flowfile_path <- system.file("extdata", "B4_18_1.fcs", package = "cyanoFilter", mustWork = TRUE) flowfile <- flowCore::read.FCS(flowfile_path, alter.names = TRUE, transformation = FALSE, emptyValue = FALSE, dataset = 1) flowfile_nona <- cyanoFilter::noNA(x = flowfile) flowfile_noneg <- cyanoFilter::noNeg(x = flowfile_nona) flowfile_logtrans <- cyanoFilter::lnTrans(x = flowfile_noneg, c('SSC.W', 'TIME')) oneDgate(flowfile, 'RED.B.HLin')