Methods for imaging mass cytometry data analysis


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Documentation for package ‘imcRtools’ version 1.0.2

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aggregateNeighbors Function to aggregate all neighbors of each cell.
binAcrossPixels Aggregate consecutive pixels per single-metal spot
buildSpatialGraph Builds an interaction graph based on the cells' locations
countInteractions Summarizes cell-cell interactions within grouping levels (e.g. images)
filterPixels Filter pixels based on their assigned masses
findBorderCells Find cells at the image border
patchDetection Function to detect patches containing defined cell types
plotSpatial Visualizes the spatial locations and interactions of cells
plotSpotHeatmap Summarizes and visualizes the pixel intensities per spot and channel
readImagefromTXT Reads one or multiple .txt files into a CytoImageList object
readSCEfromTXT Generates a SingleCellExperiment from .txt files
read_cpout Reads in single-cell data generated by the ImcSegmentationPipeline
read_steinbock Reads in single-cell data generated by the steinbock pipeline
show_cpout_features Display all features measured by CellProfiler.
testInteractions Tests if cell types interact more or less frequently than random