kallisto | bustools R utilities


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Documentation for package ‘BUSpaRse’ version 1.7.1

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.get_velocity_files Generate RNA velocity files for GRanges
annots_from_fa_df Get genome annotation from Ensembl FASTA file
annots_from_fa_GRanges Get genome annotation from Ensembl FASTA file
annot_circular Transfer information about circular chromosomes between genome and annotation
cellranger_biotypes Cell Ranger gene biotypes
check_char1 Check that an object is a character vector of length 1
check_genome Check for chromosomes in genome but not annotation
check_gff Check inputs to tr2g_gtf and tr2g_gff3
check_tag_present Check that a tag is present in attribute field of GTF/GFF
check_tx Check if transcript ID in transcriptome and annotation match
dl_transcriptome Download transcriptome from Ensembl
EC2gene Map EC Index to Genes Compatible with the EC
ensembl_gene_biotypes Gene biotypes from Ensembl
ensembl_gff_mcols These are the column names of the 'mcols' when the Ensembl GTF file is read into R as a 'GRanges', including 'gene_id', 'transcript_id', 'biotype', 'description', and so on, and the mandatory tags like 'ID', 'Name', and 'Parent'.
ensembl_gtf_mcols Tags in the attributes field of Ensembl GTF files
ensembl_tx_biotypes Transcript biotypes from Ensembl
get_inflection Plot the transposed knee plot and inflection point
get_intron_flanks Get flanked intronic ranges
get_knee_df Plot the transposed knee plot and inflection point
get_velocity_files Get files required for RNA velocity with bustools
get_velocity_files-method Get files required for RNA velocity with bustools
knee_plot Plot the transposed knee plot and inflection point
make_sparse_matrix Convert the Output of 'kallisto bus' into Gene by Gell Matrix
match_style Match chromosome naming styles of annotation and genome
read_count_output Read matrix along with barcode and gene names
read_velocity_output Read intronic and exonic matrices into R
refseq_gff_mcols Tags in the attributes field of RefSeq GFF files
save_tr2g_bustools Save transcript to gene file for use in 'bustools'
sort_tr2g Sort transcripts to the same order as in kallisto index
species2dataset Convert Latin species name to dataset name
standardize_tags Standardize GRanges field names
subset_annot Subset genome annotation
subset_annot-method Subset genome annotation
sub_annot Remove chromosomes in anotation absent from genome
tr2g_EnsDb Get transcript and gene info from EnsDb objects
tr2g_ensembl Get transcript and gene info from Ensembl
tr2g_fasta Get transcript and gene info from names in FASTA files
tr2g_gff3 Get transcript and gene info from GFF3 file
tr2g_GRanges Get transcript and gene info from GRanges
tr2g_gtf Get transcript and gene info from GTF file
tr2g_junction tr2g for exon-exon junctions
tr2g_TxDb Get transcript and gene info from TxDb objects
transcript2gene Map Ensembl transcript ID to gene ID
validate_velocity_input Validate input to get_velocity_files
write_velocity_output Write the files for RNA velocity to disk