Contents

1 Summary of the output objects of scTensor

Here, we introduced the objects saved in reanalysis.RData.

suppressPackageStartupMessages(library("scTensor"))
load("reanalysis.RData")

After performing cellCellReport, some R objects are saved in the reanalysis.RData as follows;

2 Execution of scTensor with the different options

Using the reanalysis.RData, some users may want to perform scTensor with different parameters.

For example, some users want to perform cellCellDecomp with different ranks, perform cellCellReport with omitting some enrichment analysis, provide the results to their collaborators.

To do such tasks, just type like belows.

if(!require(LRBase.Hsa.eg.db)){
    BiocManager::install("LRBase.Hsa.eg.db")
    suppressPackageStartupMessages(library(LRBase.Hsa.eg.db))
}
# Register the file pass of user's LRBase
metadata(sce)$lrbase <- dbfile(LRBase.Hsa.eg.db)
# CCI Tensor Decomposition
cellCellDecomp(sce, ranks=c(6,5), assayNames="normcounts")
# HTML Report
cellCellReport(sce, reducedDimNames="TSNE", assayNames="normcounts",
    title="Cell-cell interaction within Germline_Male, GSE86146",
    author="Koki Tsuyuzaki", html.open=TRUE,
    goenrich=TRUE, meshenrich=FALSE, reactomeenrich=FALSE,
    doenrich=FALSE, ncgenrich=FALSE, dgnenrich=FALSE)

Session information

## R version 4.1.0 (2021-05-18)
## Platform: x86_64-pc-linux-gnu (64-bit)
## Running under: Ubuntu 20.04.2 LTS
## 
## Matrix products: default
## BLAS:   /home/biocbuild/bbs-3.13-bioc/R/lib/libRblas.so
## LAPACK: /home/biocbuild/bbs-3.13-bioc/R/lib/libRlapack.so
## 
## locale:
##  [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C              
##  [3] LC_TIME=en_GB              LC_COLLATE=C              
##  [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8   
##  [7] LC_PAPER=en_US.UTF-8       LC_NAME=C                 
##  [9] LC_ADDRESS=C               LC_TELEPHONE=C            
## [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C       
## 
## attached base packages:
## [1] parallel  stats4    stats     graphics  grDevices utils     datasets 
## [8] methods   base     
## 
## other attached packages:
##  [1] AnnotationHub_3.0.0                    
##  [2] BiocFileCache_2.0.0                    
##  [3] dbplyr_2.1.1                           
##  [4] scTGIF_1.6.0                           
##  [5] Homo.sapiens_1.3.1                     
##  [6] TxDb.Hsapiens.UCSC.hg19.knownGene_3.2.2
##  [7] org.Hs.eg.db_3.13.0                    
##  [8] GO.db_3.13.0                           
##  [9] OrganismDbi_1.34.0                     
## [10] GenomicFeatures_1.44.0                 
## [11] AnnotationDbi_1.54.0                   
## [12] LRBase.Mmu.eg.db_2.0.0                 
## [13] SingleCellExperiment_1.14.0            
## [14] SummarizedExperiment_1.22.0            
## [15] Biobase_2.52.0                         
## [16] GenomicRanges_1.44.0                   
## [17] GenomeInfoDb_1.28.0                    
## [18] IRanges_2.26.0                         
## [19] S4Vectors_0.30.0                       
## [20] BiocGenerics_0.38.0                    
## [21] MatrixGenerics_1.4.0                   
## [22] matrixStats_0.58.0                     
## [23] scTensor_2.2.0                         
## [24] RSQLite_2.2.7                          
## [25] LRBase.Hsa.eg.db_2.0.0                 
## [26] LRBaseDbi_2.2.0                        
## [27] BiocStyle_2.20.0                       
## 
## loaded via a namespace (and not attached):
##   [1] Hmisc_4.5-0                   ica_1.0-2                    
##   [3] Rsamtools_2.8.0               foreach_1.5.1                
##   [5] lmtest_0.9-38                 crayon_1.4.1                 
##   [7] spatstat.core_2.1-2           MASS_7.3-54                  
##   [9] nlme_3.1-152                  backports_1.2.1              
##  [11] GOSemSim_2.18.0               MeSHDbi_1.28.0               
##  [13] rlang_0.4.11                  XVector_0.32.0               
##  [15] ROCR_1.0-11                   irlba_2.3.3                  
##  [17] nnTensor_1.0.7                filelock_1.0.2               
##  [19] GOstats_2.58.0                BiocParallel_1.26.0          
##  [21] rjson_0.2.20                  tagcloud_0.6                 
##  [23] bit64_4.0.5                   glue_1.4.2                   
##  [25] sctransform_0.3.2             spatstat.sparse_2.0-0        
##  [27] dotCall64_1.0-1               spatstat.geom_2.1-0          
##  [29] tcltk_4.1.0                   DOSE_3.18.0                  
##  [31] tidyselect_1.1.1              SeuratObject_4.0.1           
##  [33] fitdistrplus_1.1-3            XML_3.99-0.6                 
##  [35] tidyr_1.1.3                   zoo_1.8-9                    
##  [37] GenomicAlignments_1.28.0      xtable_1.8-4                 
##  [39] magrittr_2.0.1                evaluate_0.14                
##  [41] ggplot2_3.3.3                 zlibbioc_1.38.0              
##  [43] rstudioapi_0.13               miniUI_0.1.1.1               
##  [45] bslib_0.2.5.1                 rpart_4.1-15                 
##  [47] fastmatch_1.1-0               ensembldb_2.16.0             
##  [49] treeio_1.16.0                 maps_3.3.0                   
##  [51] fields_12.3                   shiny_1.6.0                  
##  [53] xfun_0.23                     cluster_2.1.2                
##  [55] tidygraph_1.2.0               TSP_1.1-10                   
##  [57] KEGGREST_1.32.0               tibble_3.1.2                 
##  [59] interactiveDisplayBase_1.30.0 ggrepel_0.9.1                
##  [61] biovizBase_1.40.0             ape_5.5                      
##  [63] listenv_0.8.0                 dendextend_1.15.1            
##  [65] Biostrings_2.60.0             png_0.1-7                    
##  [67] future_1.21.0                 withr_2.4.2                  
##  [69] bitops_1.0-7                  ggforce_0.3.3                
##  [71] RBGL_1.68.0                   plyr_1.8.6                   
##  [73] GSEABase_1.54.0               AnnotationFilter_1.16.0      
##  [75] pillar_1.6.1                  cachem_1.0.5                 
##  [77] graphite_1.38.0               vctrs_0.3.8                  
##  [79] ellipsis_0.3.2                generics_0.1.0               
##  [81] plot3D_1.3                    MeSH.Aca.eg.db_1.15.0        
##  [83] outliers_0.14                 tools_4.1.0                  
##  [85] foreign_0.8-81                entropy_1.3.0                
##  [87] munsell_0.5.0                 tweenr_1.0.2                 
##  [89] fgsea_1.18.0                  DelayedArray_0.18.0          
##  [91] fastmap_1.1.0                 compiler_4.1.0               
##  [93] abind_1.4-5                   httpuv_1.6.1                 
##  [95] rtracklayer_1.52.0            Gviz_1.36.0                  
##  [97] plotly_4.9.3                  GenomeInfoDbData_1.2.6       
##  [99] gridExtra_2.3                 lattice_0.20-44              
## [101] deldir_0.2-10                 visNetwork_2.0.9             
## [103] AnnotationForge_1.34.0        utf8_1.2.1                   
## [105] later_1.2.0                   dplyr_1.0.6                  
## [107] jsonlite_1.7.2                concaveman_1.1.0             
## [109] scales_1.1.1                  graph_1.70.0                 
## [111] tidytree_0.3.3                pbapply_1.4-3                
## [113] genefilter_1.74.0             lazyeval_0.2.2               
## [115] promises_1.2.0.1              MeSH.db_1.15.0               
## [117] latticeExtra_0.6-29           goftest_1.2-2                
## [119] spatstat.utils_2.1-0          reticulate_1.20              
## [121] checkmate_2.0.0               rmarkdown_2.8                
## [123] cowplot_1.1.1                 schex_1.6.0                  
## [125] MeSH.Syn.eg.db_1.15.0         webshot_0.5.2                
## [127] Rtsne_0.15                    dichromat_2.0-0              
## [129] BSgenome_1.60.0               uwot_0.1.10                  
## [131] igraph_1.2.6                  survival_3.2-11              
## [133] yaml_2.2.1                    plotrix_3.8-1                
## [135] htmltools_0.5.1.1             memoise_2.0.0                
## [137] VariantAnnotation_1.38.0      rTensor_1.4.8                
## [139] BiocIO_1.2.0                  Seurat_4.0.1                 
## [141] seriation_1.2-9               graphlayouts_0.7.1           
## [143] viridisLite_0.4.0             digest_0.6.27                
## [145] assertthat_0.2.1              ReactomePA_1.36.0            
## [147] mime_0.10                     rappdirs_0.3.3               
## [149] registry_0.5-1                spam_2.6-0                   
## [151] future.apply_1.7.0            misc3d_0.9-0                 
## [153] data.table_1.14.0             blob_1.2.1                   
## [155] cummeRbund_2.34.0             splines_4.1.0                
## [157] Formula_1.2-4                 ProtGenerics_1.24.0          
## [159] RCurl_1.98-1.3                hms_1.1.0                    
## [161] colorspace_2.0-1              base64enc_0.1-3              
## [163] BiocManager_1.30.15           aplot_0.0.6                  
## [165] nnet_7.3-16                   sass_0.4.0                   
## [167] Rcpp_1.0.6                    bookdown_0.22                
## [169] RANN_2.6.1                    MeSH.PCR.db_1.15.0           
## [171] enrichplot_1.12.0             fansi_0.4.2                  
## [173] parallelly_1.25.0             R6_2.5.0                     
## [175] grid_4.1.0                    ggridges_0.5.3               
## [177] lifecycle_1.0.0               curl_4.3.1                   
## [179] MeSH.Bsu.168.eg.db_1.15.0     leiden_0.3.7                 
## [181] MeSH.AOR.db_1.15.0            meshr_1.28.0                 
## [183] jquerylib_0.1.4               DO.db_2.9                    
## [185] Matrix_1.3-3                  qvalue_2.24.0                
## [187] RcppAnnoy_0.0.18              RColorBrewer_1.1-2           
## [189] iterators_1.0.13              stringr_1.4.0                
## [191] htmlwidgets_1.5.3             polyclip_1.10-0              
## [193] biomaRt_2.48.0                purrr_0.3.4                  
## [195] shadowtext_0.0.8              reactome.db_1.76.0           
## [197] mgcv_1.8-35                   globals_0.14.0               
## [199] htmlTable_2.2.1               patchwork_1.1.1              
## [201] codetools_0.2-18              prettyunits_1.1.1            
## [203] gtable_0.3.0                  DBI_1.1.1                    
## [205] highr_0.9                     tensor_1.5                   
## [207] httr_1.4.2                    KernSmooth_2.23-20           
## [209] stringi_1.6.2                 progress_1.2.2               
## [211] msigdbr_7.4.1                 reshape2_1.4.4               
## [213] farver_2.1.0                  heatmaply_1.2.1              
## [215] annotate_1.70.0               viridis_0.6.1                
## [217] hexbin_1.28.2                 fdrtool_1.2.16               
## [219] Rgraphviz_2.36.0              magick_2.7.2                 
## [221] ggtree_3.0.0                  rvcheck_0.1.8                
## [223] restfulr_0.0.13               Category_2.58.0              
## [225] scattermore_0.7               BiocVersion_3.13.1           
## [227] bit_4.0.4                     spatstat.data_2.1-0          
## [229] scatterpie_0.1.6              jpeg_0.1-8.1                 
## [231] ggraph_2.0.5                  babelgene_21.4               
## [233] pkgconfig_2.0.3               MeSH.Hsa.eg.db_1.15.0        
## [235] knitr_1.33