Contents

1 Introduction

The ambitions of collaborative single cell biology will only be achieved through the coordinated efforts of many groups, to help clarify cell types and dynamics in an array of functional and environmental contexts. The use of formal ontology in this pursuit is well-motivated and research progress has already been substantial.

Bakken et al. (2017) discuss “strategies for standardized cell type representations based on the data outputs from [high-content flow cytometry and single cell RNA sequencing], including ‘context annotations’ in the form of standardized experiment metadata about the specimen source analyzed and marker genes that serve as the most useful features in machine learning-based cell type classification models.” Aevermann et al. (2018) describe how the FAIR principles can be implemented using statistical identification of necessary and sufficient conditions for determining cell class membership. They propose that Cell Ontology can be transformed to a broadly usable knowledgebase through the incorporation of accurate marker gene signatures for cell classes.

In this vignette, we review key concepts and tasks required to make progress in the adoption and application of ontological discipline in Bioconductor-oriented data analysis.

We’ll start by setting up some package attachments and ontology objects.

2 Scope of package

The following table describes the resources available with get* commands defined in ontoProc.

X func purpose nclass nprop nroots datav fmtv
1 getCellLineOnto Cell line catalog 41780 6 18 NA NA
2 getCellOnto Cell biology concepts 6708 59 38 releases/2018-07-07 1.2
3 getCellosaurusOnto Cell line concepts 87311 6 87311 23 1.2
4 getChebiLite Chemicals of biological interest 108496 6 12 155 1.2
5 getChebiOnto 108496 33 12 155 1.2
6 getDiseaseOnto Human disease 11283 24 13 releases/2018-06-29 1.2
7 getEFOOnto Experimental factors 20115 6 36 2.87 1.2
8 getGeneOnto Gene ontology 47123 43 10 releases/2018-03-27 1.2
9 getHCAOnto Human cell atlas 11047 6 76 NA NA
10 getOncotreeOnto Tumor relations 1298 15 3 ncit/releases/2017-12-15/ncit-oncotree.ttl 1.2
11 getPATOnto Phenotypes and traits 2670 43 21 releases/2018-11-12 1.2
12 getPROnto Protein ontology 315957 6 53 57 1.2
13 getUBERON_NE Anatomy 14937 6 135 releases/2017-09-09 1.2

3 Methods

3.1 Conceptual overview of ontology with cell types

Definitions, semantics. For concreteness, we provide some definitions and examples. We use ontology to denote the systematic organization of terminology used in a conceptual domain. The Cell Ontology is a graphical data structure with carefully annotated terms as nodes and conventionally defined semantic relationships among terms serving as edges. As an example, lung ciliated cell has URI . This URI includes a fixed-length identifier CL_1000271 with unambiguous interpretation wherever it is encountered. There is a chain of relationships from lung ciliated cell up through ciliated cell, then native cell, then cell, each possessing its own URI and related interpretive metadata. The relationship connecting the more precise to the less precise term in this chain is denoted SubclassOf. Ciliated cell is equivalent to a native cell that has plasma membrane part cilium. Semantic characteristics of terms and relationships are used to infer relationships among terms that may not have relations directly specified in available ontologies.

Barriers to broad adoption. Given the wealth of material available in biological ontologies, it is somewhat surprising that formal annotation is so seldom used in practice. Barriers to more common use of ontology in data annotation include: (i) Non-existence of exact matching between intended term and terms available in ontologies of interest. (ii) The practical problem of decoding ontology identifiers. A GO tag or CL tag is excellent for programming, but it is clumsy to co-locate with the tag the associated natural language term or phrase. (iii) Likelihood of disagreement of suitability of terms for conditions observed at the boundaries of knowledge. To help cope with the first of these problems, Bioconductor’s ontologyProc package includes a function liberalMap which will search an ontology for terms lexically close to some target term or phrase. The second problem can be addressed with more elaborate data structures for variable annotation and programming in R, and the third problem will diminish in importance as the value of ontology adoption becomes manifest in more applications.

Class vs. instance. It is important to distinguish the practice of designing and maintaining ontologies from the use of ontological class terms to annotate instances of the concepts. The combination of an ontology and a set of annotated instances is called a knowledge base. To illustrate some of the salient distinctions here, consider the cell line called A549, which is established from a human lung adenocarcinoma sample. There is no mention of A549 in the Cell Ontology. However, A549 is present in the EBI Experimental Factor Ontology as a subclass of the “Homo sapiens cell line” class. Presumably this is because A549 is a class of cells that are widely used experimentally, and this cell line constitutes a concept deserving of mapping in the universe of experimental factors. In the universe of concepts related to cell structure and function per se, A549 is an individual that can be characterized through possession of or lack of properties enumerated in Cell Ontology, but it is not deserving of inclusion in that ontology.

3.2 Illustration in a single-cell RNA-seq dataset

The 10X Genomics corporation has distributed a dataset on results of sequencing 10000 PBMC from a healthy donor . Subsets of the data are used in tutorials for the Seurat analytical suite (Butler et al. (2018)).

3.2.1 Labeling PBMC in the Seurat tutorial

One result of the tutorial analysis of the 3000 cell subset is a table of cell types and expression-based markers of cell identity. The first three columns of the table below are from concluding material in the Seurat tutorial; the remaining columns are created by “manual” matching between the Seurat terms and terms found in Cell Ontology.

grp markers seurTutType formal tag
0 IL7R CD4 T cells CD4-positive helper T cell CL:0000492
1 CD14, LYZ CD14+ Monocytes CD14-positive monocyte CL:0001054
2 MS4A1 B cells B cell CL:0000236
3 CD8A CD8 T cells CD8-positive, alpha-beta T cell CL:0000625
4 FCGR3A, MS4A7 FCGR3A+ Monocytes monocyte CL:0000576
5 GNLY, NKG7 NK cells natural killer cell CL:0000623
6 FCER1A, CST3 Dendritic Cells dendritic cell CL:0000451
7 PPBP Megakaryocytes megakaryocyte CL:0000556

3.2.2 Relationships asserted in the Cell Ontology

Given the informally selected tags in the table above, we can sketch the Cell Ontology graph connecting the associated cell types. The ontoProc package adds functionality to ontologyPlot with make_graphNEL_from_ontology_plot. This allows use of all Rgraphviz and igraph visualization facilities for graphs derived from ontology structures.

Here we display the PBMC cell sets reported in the Seurat tutorial.

3.2.3 Molecular features asserted in the Cell Ontology

The CLfeats function traces relationships and properties from a given Cell Ontology class. Briefly, each class can assert that it is the intersection_of other classes, and has_part, lacks_part, has_plasma_membrane_part, lacks_plasma_membrane_part can be asserted as relationships holding between cell type instances and cell components. The components are often cross-referenced to Protein Ontology or Gene Ontology. When the Protein Ontology component has a synonym for which an HGNC symbol is provided, that symbol is retrieved by CLfeats. Here we obtain the listing for a mature CD1a-positive dermal dendritic cell.

tag prtag cond entity SYMBOL name
CL:0000990 PR:000001013 hiPMAmt integrin alpha-X ITGAX conventional dendritic cell
CL:0000451 PR:000001002 lacksPMP CD19 molecule CD19 dendritic cell
CL:0000451 PR:000001003 lacksPMP CD34 molecule CD34 dendritic cell
CL:0000451 PR:000001020 lacksPMP CD3 epsilon CD3E dendritic cell
CL:0000451 PR:000001024 lacksPMP neural cell adhesion molecule 1 NCAM1 dendritic cell
CL:0000451 PR:000001289 lacksPMP membrane-spanning 4-domains subfamily A member 1 MS4A1 dendritic cell
CL:0000451 GO:0042613 hasPart MHC class II protein complex NA dendritic cell
CL:0000841 GO:0042613 hiPMAmt MHC class II protein complex NA mature conventional dendritic cell
CL:0000841 PR:000001412 hiPMAmt CD86 molecule CD86 mature conventional dendritic cell
CL:0000841 PR:000001438 hiPMAmt CD80 molecule CD80 mature conventional dendritic cell
CL:0000000 GO:0005634 hasPart nucleus NA cell
CL:0000000 GO:0005634 hasPart nucleus NA cell

The ctmarks function starts a shiny app that generates tables of this sort for selected cell types.

ctmarks snapshot

ctmarks snapshot

3.2.4 Mapping from gene ‘presence/role’ to cell type

The sym2CellOnto function helps find mention of given gene symbols in properties or parts of cell types.

sym cond cl type
ITGAM lacksPMP CL:0000037 hematopoietic stem cell
ITGAM lacksPMP CL:0000547 proerythroblast
ITGAM lacksPMP CL:0000553 megakaryocyte progenitor cell
ITGAM lacksPMP CL:0000558 reticulocyte
ITGAM lacksPMP CL:0000611 eosinophil progenitor cell
ITGAM lacksPMP CL:0000613 basophil progenitor cell
ITGAM lacksPMP CL:0000765 erythroblast
ITGAM lacksPMP CL:0000831 mast cell progenitor
ITGAM lacksPMP CL:0000836 promyelocyte
ITGAM lacksPMP CL:0000837 hematopoietic multipotent progenitor cell
ITGAM lacksPMP CL:0000872 splenic marginal zone macrophage
ITGAM lacksPMP CL:0000941 thymic conventional dendritic cell
ITGAM lacksPMP CL:0000942 thymic plasmacytoid dendritic cell
ITGAM lacksPMP CL:0000989 CD11c-low plasmacytoid dendritic cell
ITGAM lacksPMP CL:0000998 CD8_alpha-negative CD11b-negative dendritic cell
ITGAM lacksPMP CL:0001000 CD8_alpha-positive CD11b-negative dendritic cell
ITGAM lacksPMP CL:0001029 common dendritic progenitor
ITGAM lacksPMP CL:0001060 hematopoietic oligopotent progenitor cell, lineage-negative
ITGAM lacksPMP CL:0001066 erythroid progenitor cell, mammalian
ITGAM lacksPMP CL:0002010 pre-conventional dendritic cell
ITGAM lacksPMP CL:0002089 group 2 innate lymphoid cell, mouse
ITGAM lacksPMP CL:0002679 natural helper lymphocyte
ITGAM hiPMAmt CL:0000581 peritoneal macrophage
ITGAM hiPMAmt CL:0002347 CD27-high, CD11b-high natural killer cell, mouse
ITGAM hiPMAmt CL:0002348 CD27-low, CD11b-high natural killer cell, mouse
ITGAM hiPMAmt CL:0002474 lymphoid MHC-II-negative classical monocyte
ITGAM hiPMAmt CL:0002475 lymphoid MHC-II-negative non-classical monocyte
ITGAM hiPMAmt CL:0002505 liver CD103-negative dendritic cell
ITGAM hiPMAmt CL:0002510 CD103-negative, langerin-positive lymph node dendritic cell
ITGAM hiPMAmt CL:0002512 CD11b-high, CD103-negative, langerin-negative lymph node dendritic cell
ITGAM loPMAmt CL:0000091 Kupffer cell
ITGAM loPMAmt CL:0000583 alveolar macrophage
ITGAM loPMAmt CL:0000868 lymph node macrophage
ITGAM loPMAmt CL:0000874 splenic red pulp macrophage
ITGAM loPMAmt CL:0002345 CD27-low, CD11b-low immature natural killer cell, mouse
ITGAM loPMAmt CL:0002349 CD27-high, CD11b-low natural killer cell, mouse
ITGAM loPMAmt CL:0002506 liver CD103-positive dendritic cell
ITGAM loPMAmt CL:0002509 CD103-positive, langerin-positive lymph node dendritic cell
ITGAM loPMAmt CL:0002511 CD11b-low, CD103-negative, langerin-negative lymph node dendritic cell 
## 
##  hiPMAmt lacksPMP  loPMAmt 
##        8       22        9
sym cond cl type
FOXP3 hasPart CL:0000902 induced T-regulatory cell
FOXP3 hasPart CL:0000903 natural T-regulatory cell
FOXP3 hasPart CL:0000919 CD8-positive, CD25-positive, alpha-beta regulatory T cell
FOXP3 hasPart CL:0000920 CD8-positive, CD28-negative, alpha-beta regulatory T cell

3.3 Adding terms to ontology_index structures to ‘extend’ Cell Ontology

The task of extending an ontology is partly bureaucratic in nature and depends on a collection of endorsements and updates to centralized information structures. In order to permit experimentation with interfaces and new content that may be quite speculative, we include an approach to combining new ontology ‘terms’ of structure similar to those endorsed in Cell Ontology, to ontologyIndex-based ontology_index instances.

3.3.1 Use case: a set of cell types defined by “diagonal expression”

For a demonstration, we consider the discussion in Bakken et al. (2017), of a ‘diagonal’ expression pattern defining a group of novel cell types. A set of genes is identified and cells are distinguised by expressing exactly one gene from the set.

Diagonal expression pattern.

Diagonal expression pattern.

The necessary information is collected in a vector. The vector is the set of genes, the name of element i is the tag to be associated with the type of cell that expresses gene i and does not express any other gene in the set.

3.3.2 A data.frame defining the cell types and their properties

The cyclicSigset function produces a data.frame instance connecting cell types with the genes expressed or unexpressed.

## [1] 100   3
##        gene   type     cond
## 1  ARHGEF28 CL:X09   hasExp
## 2     GRIK3 CL:X09 lacksExp
## 3     NTNG1 CL:X09 lacksExp
## 4     BAGE2 CL:X09 lacksExp
## 5      MC4R CL:X09 lacksExp
## 9      SNCG CL:X09 lacksExp
## 10      EGF CL:X09 lacksExp
## 11    BAGE2 CL:X03   hasExp
## 12    GRIK3 CL:X03 lacksExp
## 13    NTNG1 CL:X03 lacksExp
## 
##   hasExp lacksExp 
##       10       90

It is expected that a tabular layout like this will suffice to handle general situations of cell type definition.

3.3.3 Translating the data.frame elements to OBO Term instances

The most complicated aspect of novel OBO term construction is the proper specifications of relationships with existing ontology components. A prolog that is mostly shared by all terms is generated programmatically for the diagonal pattern task.

The ldfToTerms API uses this to create a set of strings that can be parsed as a term.

##  [1] "[Term]"                                                                                                        
##  [2] "id: CL:X01"                                                                                                    
##  [3] "name: GRIK3-expressing cortical layer 1 interneuron, human"                                                    
##  [4] "def: 'GRIK3-expressing cortical layer 1 interneuron, human described via RNA-seq observations' [PMID 29322913]"
##  [5] "is_a: CL:0000099 ! interneuron"                                                                                
##  [6] "intersection_of: CL:0000099 ! interneuron"                                                                     
##  [7] "has_expression_of: PR:000008242 ! GRIK3"                                                                       
##  [8] "lacks_expression_of: PR:000011467 ! NTNG1"                                                                     
##  [9] "lacks_expression_of: PR:000004625 ! BAGE2"                                                                     
## [10] "lacks_expression_of: PR:000001237 ! MC4R"                                                                      
## [11] "lacks_expression_of: PR:000012318 ! PAX6"                                                                      
## [12] "lacks_expression_of: PR:000016738 ! TSPAN12"                                                                   
## [13] "lacks_expression_of: PR:B8ZZ34 ! hSHISA8"                                                                      
## [14] "lacks_expression_of: PR:000015325 ! SNCG"                                                                      
## [15] "lacks_expression_of: PR:000013942 ! ARHGEF28"                                                                  
## [16] "lacks_expression_of: PR:000006928 ! EGF"                                                                       
## [17] "[Term]"                                                                                                        
## [18] "id: CL:X02"                                                                                                    
## [19] "name: NTNG1-expressing cortical layer 1 interneuron, human"                                                    
## [20] "def: 'NTNG1-expressing cortical layer 1 interneuron, human described via RNA-seq observations' [PMID 29322913]"

The content in tms can then be appended to the content of the Cell Ontology cl.obo as text for import with ontologyIndex::get_OBO.

3.4 Subsetting SingleR resources using ontological mapping

3.4.1 A data.frame mapping from informal to formal terms

Aaron Lun has produced a mapping from informal terms used in the Human Primary Cell Atlas to Cell Ontology tags. We provisionally include a copy of this mapping in ontoProc:

##                          uncontrolled controlled
## 1        DC:monocyte-derived:immature CL:0000840
## 2      DC:monocyte-derived:Galectin-1 CL:0000451
## 3             DC:monocyte-derived:LPS CL:0000451
## 4                 DC:monocyte-derived CL:0000451
## 5 Smooth_muscle_cells:bronchial:vit_D CL:0002598
## 6       Smooth_muscle_cells:bronchial CL:0002598

We will rename columns of this map for convenience of our bind_formal_tags method.

3.4.2 Binding formal tags to the HPCA data

I am turning this code off for now because there is no standard approach to getting the mapping from the SummarizedExperment yet. When SingleR merges the ‘standardized’ branch, this will come back.

Let’s retrieve the HPCA data from SingleR:

## Loading required package: SummarizedExperiment
## Loading required package: MatrixGenerics
## Loading required package: matrixStats
## 
## Attaching package: 'MatrixGenerics'
## The following objects are masked from 'package:matrixStats':
## 
##     colAlls, colAnyNAs, colAnys, colAvgsPerRowSet, colCollapse,
##     colCounts, colCummaxs, colCummins, colCumprods, colCumsums,
##     colDiffs, colIQRDiffs, colIQRs, colLogSumExps, colMadDiffs,
##     colMads, colMaxs, colMeans2, colMedians, colMins, colOrderStats,
##     colProds, colQuantiles, colRanges, colRanks, colSdDiffs, colSds,
##     colSums2, colTabulates, colVarDiffs, colVars, colWeightedMads,
##     colWeightedMeans, colWeightedMedians, colWeightedSds,
##     colWeightedVars, rowAlls, rowAnyNAs, rowAnys, rowAvgsPerColSet,
##     rowCollapse, rowCounts, rowCummaxs, rowCummins, rowCumprods,
##     rowCumsums, rowDiffs, rowIQRDiffs, rowIQRs, rowLogSumExps,
##     rowMadDiffs, rowMads, rowMaxs, rowMeans2, rowMedians, rowMins,
##     rowOrderStats, rowProds, rowQuantiles, rowRanges, rowRanks,
##     rowSdDiffs, rowSds, rowSums2, rowTabulates, rowVarDiffs, rowVars,
##     rowWeightedMads, rowWeightedMeans, rowWeightedMedians,
##     rowWeightedSds, rowWeightedVars
## Loading required package: GenomicRanges
## Loading required package: stats4
## Loading required package: BiocGenerics
## Loading required package: parallel
## 
## Attaching package: 'BiocGenerics'
## The following objects are masked from 'package:parallel':
## 
##     clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
##     clusterExport, clusterMap, parApply, parCapply, parLapply,
##     parLapplyLB, parRapply, parSapply, parSapplyLB
## The following objects are masked from 'package:stats':
## 
##     IQR, mad, sd, var, xtabs
## The following objects are masked from 'package:base':
## 
##     Filter, Find, Map, Position, Reduce, anyDuplicated, append,
##     as.data.frame, basename, cbind, colnames, dirname, do.call,
##     duplicated, eval, evalq, get, grep, grepl, intersect, is.unsorted,
##     lapply, mapply, match, mget, order, paste, pmax, pmax.int, pmin,
##     pmin.int, rank, rbind, rownames, sapply, setdiff, sort, table,
##     tapply, union, unique, unsplit, which.max, which.min
## Loading required package: S4Vectors
## 
## Attaching package: 'S4Vectors'
## The following objects are masked from 'package:base':
## 
##     I, expand.grid, unname
## Loading required package: IRanges
## Loading required package: GenomeInfoDb
## Loading required package: Biobase
## Welcome to Bioconductor
## 
##     Vignettes contain introductory material; view with
##     'browseVignettes()'. To cite Bioconductor, see
##     'citation("Biobase")', and for packages 'citation("pkgname")'.
## 
## Attaching package: 'Biobase'
## The following object is masked from 'package:MatrixGenerics':
## 
##     rowMedians
## The following objects are masked from 'package:matrixStats':
## 
##     anyMissing, rowMedians
## snapshotDate(): 2021-05-05
## see ?celldex and browseVignettes('celldex') for documentation
## loading from cache
## see ?celldex and browseVignettes('celldex') for documentation
## loading from cache

Now bind the formal tags:

## [1] 66

We don’t check for failed mappings:

## [1] 2
## DataFrame with 2 rows and 3 columns
##            label.main             label.fine   label.ont
##           <character>            <character> <character>
## GSM154081     T_cells T_cell:CCR10+CLA+1,2..          NA
## GSM154084     T_cells T_cell:CCR10-CLA+1,2..          NA
## [1] 50

3.4.3 Subsetting using the class hierarchy of Cell Ontology

## 
##                          Monocyte                    Monocyte:CD14+ 
##                                27                                 3 
##                    Monocyte:CD16+                    Monocyte:CD16- 
##                                 6                                 7 
##                    Monocyte:CXCL4   Monocyte:F._tularensis_novicida 
##                                 2                                 6 
##                     Monocyte:MCSF Monocyte:S._typhimurium_flagellin 
##                                 2                                 1 
##             Monocyte:anti-FcgRIIB           Monocyte:leukotriene_D4 
##                                 2                                 4
## 
## CL:0000576 CL:0001054 
##         57          3
## 
##                    T_cell:CD4+              T_cell:CD4+_Naive 
##                             12                              6 
##     T_cell:CD4+_central_memory    T_cell:CD4+_effector_memory 
##                              5                              4 
##                    T_cell:CD8+     T_cell:CD8+_Central_memory 
##                             16                              3 
##    T_cell:CD8+_effector_memory T_cell:CD8+_effector_memory_RA 
##                              4                              4 
##              T_cell:CD8+_naive              T_cell:Treg:Naive 
##                              4                              2 
##                T_cell:effector             T_cell:gamma-delta 
##                              4                              2
## 
## CL:0000624 CL:0000625 CL:0000798 CL:0000895 CL:0000900 CL:0000904 CL:0000905 
##         12         16          2          6          4          5          4 
## CL:0000907 CL:0000911 CL:0000913 CL:0001062 CL:0002677 
##          3          4          4          4          2

References

Aevermann, Brian D., Mark Novotny, Trygve Bakken, Jeremy A. Miller, Alexander D. Diehl, David Osumi-Sutherland, Roger S. Lasken, Ed S. Lein, and Richard H. Scheuermann. 2018. “Cell type discovery using single-cell transcriptomics: Implications for ontological representation.” Human Molecular Genetics 27 (R1): R40–R47. https://doi.org/10.1093/hmg/ddy100.

Bakken, Trygve, Lindsay Cowell, Brian D. Aevermann, Mark Novotny, Rebecca Hodge, Jeremy A. Miller, Alexandra Lee, et al. 2017. “Cell type discovery and representation in the era of high-content single cell phenotyping.” BMC Bioinformatics 18 (Suppl 17). https://doi.org/10.1186/s12859-017-1977-1.

Butler, Andrew, Paul Hoffman, Peter Smibert, Efthymia Papalexi, and Rahul Satija. 2018. “Integrating Single-Cell Transcriptomic Data Across Different Conditions, Technologies, and Species.” Nature Biotechnology. https://doi.org/10.1038/nbt.4096.

Westbury, Sarah K., Ernest Turro, Daniel Greene, Claire Lentaigne, Anne M. Kelly, Tadbir K. Bariana, Ilenia Simeoni, et al. 2015. “Human Phenotype Ontology Annotation and Cluster Analysis to Unravel Genetic Defects in 707 Cases with Unexplained Bleeding and Platelet Disorders.” Genome Medicine 7 (1): 36. https://doi.org/10.1186/s13073-015-0151-5.