Application of high-throughput sequencing of T and B lymphocyte antigen receptors has great potential for improving the monitoring of lymphoid malignancies, assessing immune reconstitution after hematopoietic stem cell transplantation, and characterizing the composition of lymphocyte repertoires (Warren, E. H. et al. Blood 2013;122:19–22). LymhoSeq is an R package designed to import, analyze, and visualize antigen receptor sequencing from Adaptive Biotechnologies’ ImmunoSEQ assay. The package is also adaptable to the analysis of T and B cell receptor sequencing processed using other platforms such as MiXCR or IMGT/HighV-QUEST. This vignette has been written to highlight some of the features of LymphoSeq and guide the user through a typical workflow.

Importing data

The LymphoSeq function readImmunoSeq</