InPAS: a bioconductor package for the identification of novel alternative PolyAdenylation Sites (PAS) using RNA-seq data


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Documentation for package ‘InPAS’ version 1.22.0

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InPAS-package alternative polyadenylation and cleavage estimations
$-method Class 'UTR3eSet'
$<--method Class 'UTR3eSet'
coverageFromBedGraph read coverage from bedGraph files
coverageRate coverage rate of genes and 3UTRs
covThreshold calculate the cutoff threshold of coverage
CPsites predict the cleavage and polyadenylation(CP) site
CPsite_estimation estimate the cpsites
depthWeight calculate the depth weight for each example
distalAdj adjust distal CP sites by cleanUpdTSeq
filterRes filter results
fisher.exact.test do fisher exact test for two group datasets
get.regions.coverage claculate coverage for giving region
getCov extract coverage from bedgraph file
getUTR3eSet prepare dataset for test
getUTR3region extract long and short 3UTR region
InPAS alternative polyadenylation and cleavage estimations
inPAS do estimation of alternative polyadenylation and cleavage site in one step
lastCDSusage extract coverage of last CDS exon region
limmaAnalyze use limma to analyze the PDUI
optimalSegmentation calculate SSE
PAscore calculate the CP score
PAscore2 calculate the CP score
polishCPs polish the searching results of CP sites
prepare4GSEA prepare the files for GSEA analysis
proximalAdj adjust the proximal CP sites
proximalAdjByCleanUpdTSeq adjust the proximal CP sites by cleanUpdTseq
proximalAdjByPWM adjust the proximal CP sites by PWM
removeUTR3__UTR3 remove the candicates LIKE UTR3___UTR3
searchDistalCPs search distal CP sites
searchProximalCPs search proximal CPsites
seqLen get sequence lengths
singleGroupAnalyze do analysis for single group samples
singleSampleAnalyze do analysis for single sample
sortGR sort GRanges
testUsage do test for dPDUI
totalCoverage total coverage
trimSeqnames trim the sequence names
usage4plot prepare coverage data and fitting data for plot
utr3.danRer10 3'UTR annotation for danRer10 obtained from utr3Annotation
utr3.hg19 3'UTR annotation for hg19 obtained from utr3Annotation
utr3.mm10 3'UTR annotation for mm10 obtained from utr3Annotation
utr3Annotation extract 3'UTR from TxDb object
UTR3eSet Class 'UTR3eSet'
UTR3eSet-class Class 'UTR3eSet'
UTR3TotalCoverage extract coverage of 3UTR for CP sites prediction
UTR3usage calculate the usage of long and short form of UTR3
utr3UsageEstimation estimation of 3'UTR usage for each region
valley get the local minimal square standard error (SSE)
zScoreThrethold calculate local background cutoff value