measureF {CONFESS} | R Documentation |
It estimates the foreground signal for an identified spot or for a predefined area within the capture site.
measureF(img, area, BFarea)
img |
Data matrix. The matrix of image data from a channel. |
area |
Data matrix. The bright (spot) coordinates around the spot center. |
BFarea |
Integer. Defines a rectangular pseudo-spot size whose fluorescence will be estimated. |
The foreground (spot) signal estimates