Melting temperature

The melting temperature is the temperature at which 50% of the primer concentration are found in the form of the primer-template duplex. Hence, the melting temperature indicates the stability of primer-template duplexes. High melting temperatures indicate energetically favorable primer-template interactions, while low melting temperatures indicate low affinities. Melting temperature is of concern for PCR for several reasons:

Typical melting temperatures lie between 55 and 65 degrees Celsius. openPrimeR uses MELTING for the computation of melting temperatures.