EasyqpcR-package |
Functions to analyse real-time quantitative PCR data at IRTOMIT-INSERM U1082 |
badCt |
Evaluation of the qPCR technical replicates |
calData |
Calculation of calibration factors |
EasyqpcR |
Functions to analyse real-time quantitative PCR data at IRTOMIT-INSERM U1082 |
Efficiency_calculation |
Raw data for primer amplification efficiency calculation. |
Gene_maximisation |
A data frame containing all the data from four different qPCR runs. |
Gene_maximisation_cor |
A data frame containing all the data from four different qPCR runs. |
nrmData |
Determination of the NF, RQ, NRQ, NRQ scaled to control and their SE and SD. |
qPCR_run1 |
Raw data from the first qPCR run. |
qPCR_run2 |
Raw data from the second qPCR run. |
qPCR_run3 |
Raw data from the third qPCR run. |
slope |
Function to calculate the amplification efficiency |
totData |
Aggregation of qPCR biological replicates and data transformation |