ddCt

This package is for version 2.8 of Bioconductor; for the stable, up-to-date release version, see ddCt.

The ddCt Algorithm for the Analysis of Quantitative Real-Time PCR (qRT-PCR)

Bioconductor version: 2.8

The Delta-Delta-Ct (ddCt) Algorithm is an approximation method to determine relative gene expression with quantitative real-time PCR (qRT-PCR) experiments. Compared to other approaches, it requires no standard curve for each primer-target pair, therefore reducing the working load and yet returning accurate enough results as long as the assumptions of the amplification efficiency hold. The ddCt package implements a pipeline to collect, analyse and visualize qRT-PCR results, for example those from TaqMan SDM software, mainly using the ddCt method. The pipeline can be either invoked by a script in command-line or through the API consisting of S4-Classes, methods and functions.

Author: Jitao David Zhang, Rudolf Biczok and Markus Ruschhaupt

Maintainer: Jitao David Zhang <j.zhang at dkfz.de>

Installation

To install this package, start R and enter:

## try http:// if https:// URLs are not supported
source("https://bioconductor.org/biocLite.R")
biocLite("ddCt")

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("ddCt")

Details

Package Archives

Follow Installation instructions to use this package in your R session.