1 Introduction

1.1 Installation

rols is a Bioconductor package and should hence be installed using the dedicated functionality

## try http:// if https:// URLs are not supported
if (!requireNamespace("BiocManager", quietly=TRUE))
    install.packages("BiocManager")
BiocManager::install("rols")

1.2 Getting help

To get help, either post your question on the Bioconductor support site or open an issue on the rols github page.

1.3 The resource

The Ontology Lookup Service (OLS) [1, 2] is originally spin-off of the PRoteomics IDEntifications database (PRIDE) service, located at the EBI, and is now developed and maintained by the Samples, Phenotypes and Ontologies team at EMBL-EBI.

1.4 The package

The OLS provides a REST interface to hundreds of ontologies from a single location with a unified output format. The rols package make this possible from within R. Do do so, it relies on the httr package to query the REST interface, and access and retrieve data.

There are 223 ontologies available in the OLS, listed in the table below. Their name is to be use to defined which ontology to query.

2 A Brief rols overview

The rols package is build around a few classes that enable to query the OLS and retrieve, store and manipulate data. Each of these classes are described in more details in their respective manual pages. We start by loading the package.

library("rols")

2.1 Ontologies

The Ontology and Ontologies classes can store information about single of multiple ontologies. The latter can be easily subset using [ and [[, as one would for lists.

ol <- Ontologies()
ol
## Object of class 'Ontologies' with 223 entries
##    AERO, BCGO ... REX, UBERON
head(olsNamespace(ol))
##    aero    bcgo     cco    cdao    ceph   chebi 
##  "aero"  "bcgo"   "cco"  "cdao"  "ceph" "chebi"
ol[["go"]]
## Ontology: Gene Ontology (go)  
##   An ontology for describing the function of genes and gene products
##    Loaded: 2018-12-31 Updated: 2019-01-04 Version: 2018-12-28 
##    49769 terms  60 properties  0 individuals

It is also possible to initialise a single ontology

go <- Ontology("go")
go
## Ontology: Gene Ontology (go)  
##   An ontology for describing the function of genes and gene products
##    Loaded: 2018-12-31 Updated: 2019-01-04 Version: 2018-12-28 
##    49769 terms  60 properties  0 individuals

2.2 Terms

Single ontology terms are stored in Term objects. When more terms need to be manipulated, they are stored as Terms objects. It is easy to obtain all terms of an ontology of interest, and the resulting Terms object can be subset using [ and [[, as one would for lists.

gotrms <- terms(go) ## or terms("go")
gotrms
## Object of class 'Terms' with 49770 entries
##  From the GO ontology
##   GO:0005230, GO:0015276 ... GO:0032957, GO:0032942
gotrms[1:10]
## Object of class 'Terms' with 10 entries
##  From the GO ontology
##   GO:0005230, GO:0015276 ... GO:0001819, GO:0044831
gotrms[["GO:0090575"]]
## A Term from the GO ontology: GO:0090575 
##  Label: RNA polymerase II transcription factor complex
##   A transcription factor complex that acts at a regulatory region of a
##   gene transcribed by RNA polymerase II.

It is also possible to initialise a single term

trm <- term(go, "GO:0090575")
termId(trm)
## [1] "GO:0090575"
termLabel(trm)
## [1] "RNA polymerase II transcription factor complex"
strwrap(termDesc(trm))
## [1] "A transcription factor complex that acts at a regulatory region of a"
## [2] "gene transcribed by RNA polymerase II."

It is then possible to extract the ancestors, descendants, parents and children terms. Each of these functions return a Terms object

parents(trm)
## Object of class 'Terms' with 1 entries
##  From the GO ontology
## GO:0044798
children(trm)
## Object of class 'Terms' with 41 entries
##  From the GO ontology
##   GO:0062071, GO:0008230 ... GO:0034718, GO:0030232

Similarly, the partOf and derivesFrom functions return, for an input term, the terms it is a part of and derived from.

Finally, a single term or terms object can be coerced to a data.frame using as(x, "data.frame").

2.3 Properties

Properties (relationships) of single or multiple terms or complete ontologies can be queries with the properties method, as briefly illustrated below.

trm <- term("uberon", "UBERON:0002107")
trm
## A Term from the UBERON ontology: UBERON:0002107 
##  Label: liver
##   An exocrine gland which secretes bile and functions in metabolism of
##   protein and carbohydrate and fat, synthesizes substances involved in
##   the clotting of the blood, synthesizes vitamin A, detoxifies
##   poisonous substances, stores glycogen, and breaks down worn-out
##   erythrocytes[GO].
p <- properties(trm)
p
## Object of class 'Properties' with 112 entries
##  From the UBERON ontology
##   digestive system gland, abdomen element ... liver lobule, hepatobiliary system
p[[1]]
## A Property from the UBERON ontology: UBERON:0006925 
##  Label: digestive system gland
termLabel(p[[1]])
## [1] "digestive system gland"

3 Use case

A researcher might be interested in the trans-Golgi network. Searching the OLS is assured by the OlsSearch and olsSearch classes/functions. The first step is to defined the search query with OlsSearch, as shown below. This creates an search object of class OlsSearch that stores the query and its parameters. In records the number of requested results (default is 20) and the total number of possible results (there are 15318 results across all ontologies, in this case). At this stage, the results have not yet been downloaded, as shown by the 0 responses.

OlsSearch(q = "trans-golgi network")
## Object of class 'OlsSearch':
##   query: trans-golgi network 
##   requested: 20 (out of 15318)
##   response(s): 0

15318 results are probably too many to be relevant. Below we show how to perform an exact search by setting exact = TRUE, and limiting the search the the GO ontology by specifying ontology = "GO", or doing both.

OlsSearch(q = "trans-golgi network", exact = TRUE)
## Object of class 'OlsSearch':
##   query: trans-golgi network 
##   requested: 20 (out of 8)
##   response(s): 0
OlsSearch(q = "trans-golgi network", ontology = "GO")
## Object of class 'OlsSearch':
##   ontolgy: GO 
##   query: trans-golgi network 
##   requested: 20 (out of 756)
##   response(s): 0
OlsSearch(q = "trans-golgi network", ontology = "GO", exact = TRUE)
## Object of class 'OlsSearch':
##   ontolgy: GO 
##   query: trans-golgi network 
##   requested: 20 (out of 1)
##   response(s): 0

One case set the rows argument to set the number of desired results.

OlsSearch(q = "trans-golgi network", ontology = "GO", rows = 200)
## Object of class 'OlsSearch':
##   ontolgy: GO 
##   query: trans-golgi network 
##   requested: 200 (out of 756)
##   response(s): 0

Alternatively, one can call the allRows function to request all results.

(tgnq <- OlsSearch(q = "trans-golgi network", ontology = "GO"))
## Object of class 'OlsSearch':
##   ontolgy: GO 
##   query: trans-golgi network 
##   requested: 20 (out of 756)
##   response(s): 0
(tgnq <- allRows(tgnq))
## Object of class 'OlsSearch':
##   ontolgy: GO 
##   query: trans-golgi network 
##   requested: 756 (out of 756)
##   response(s): 0

Let’s proceed with the exact search and retrieve the results. Even if we request the default 20 results, only the 8 relevant result will be retrieved. The olsSearch function updates the previously created object (called qry below) by adding the results to it.

qry <- OlsSearch(q = "trans-golgi network", exact = TRUE)
(qry <- olsSearch(qry))
## Object of class 'OlsSearch':
##   query: trans-golgi network 
##   requested: 20 (out of 8)
##   response(s): 8

We can now transform this search result object into a fully fledged Terms object or a data.frame.

(qtrms <- as(qry, "Terms"))
## Object of class 'Terms' with 8 entries
##  From  8 ontologies
##   NCIT:C33802, OMIT:0020822 ... GO:0005802, GO:0005802
str(qdrf <- as(qry, "data.frame"))
## 'data.frame':    8 obs. of  10 variables:
##  $ id                  : chr  "ncit:class:http://purl.obolibrary.org/obo/NCIT_C33802" "omit:class:http://purl.obolibrary.org/obo/OMIT_0020822" "go:class:http://purl.obolibrary.org/obo/GO_0005802" "cco:http://purl.obolibrary.org/obo/GO_0005802" ...
##  $ iri                 : chr  "http://purl.obolibrary.org/obo/NCIT_C33802" "http://purl.obolibrary.org/obo/OMIT_0020822" "http://purl.obolibrary.org/obo/GO_0005802" "http://purl.obolibrary.org/obo/GO_0005802" ...
##  $ short_form          : chr  "NCIT_C33802" "OMIT_0020822" "GO_0005802" "GO_0005802" ...
##  $ obo_id              : chr  "NCIT:C33802" "OMIT:0020822" "GO:0005802" "GO:0005802" ...
##  $ label               : chr  "Trans-Golgi Network" "trans-Golgi Network" "trans-Golgi network" "trans-Golgi network" ...
##  $ description         :List of 8
##   ..$ : chr "A network of membrane components where vesicles bud off the Golgi apparatus to bring proteins, membranes and ot"| __truncated__
##   ..$ : NULL
##   ..$ : chr "The network of interconnected tubular and cisternal structures located within the Golgi apparatus on the side d"| __truncated__
##   ..$ : chr "The network of interconnected tubular and cisternal structures located within the Golgi apparatus on the side d"| __truncated__
##   ..$ : chr "The network of interconnected tubular and cisternal structures located within the Golgi apparatus on the side d"| __truncated__
##   ..$ : chr "The network of interconnected tubular and cisternal structures located within the Golgi apparatus on the side d"| __truncated__
##   ..$ : chr "The network of interconnected tubular and cisternal structures located within the Golgi apparatus on the side d"| __truncated__
##   ..$ : chr "The network of interconnected tubular and cisternal structures located within the Golgi apparatus on the side d"| __truncated__
##  $ ontology_name       : chr  "ncit" "omit" "go" "cco" ...
##  $ ontology_prefix     : chr  "NCIT" "OMIT" "GO" "CCO" ...
##  $ type                : chr  "class" "class" "class" "class" ...
##  $ is_defining_ontology: logi  TRUE TRUE TRUE FALSE FALSE FALSE ...

In this case, we can see that we actually retrieve the same term used across different ontologies. In such cases, it might be useful to keep only non-redundant term instances. Here, this would have been equivalent to searching the ncit, omit, go ontology

qtrms <- unique(qtrms)
termOntology(qtrms)
##  NCIT:C33802 OMIT:0020822   GO:0005802 
##       "ncit"       "omit"         "go"
termNamespace(qtrms)
## $`NCIT:C33802`
## NULL
## 
## $`OMIT:0020822`
## NULL
## 
## $`GO:0005802`
## [1] "cellular_component"

Below, we execute the same query using the GO.db package.

library("GO.db")
GOTERM[["GO:0005802"]]
## GOID: GO:0005802
## Term: trans-Golgi network
## Ontology: CC
## Definition: The network of interconnected tubular and cisternal
##     structures located within the Golgi apparatus on the side distal
##     to the endoplasmic reticulum, from which secretory vesicles
##     emerge. The trans-Golgi network is important in the later stages
##     of protein secretion where it is thought to play a key role in
##     the sorting and targeting of secreted proteins to the correct
##     destination.
## Synonym: Golgi trans face
## Synonym: Golgi trans-face
## Synonym: late Golgi
## Synonym: maturing face
## Synonym: TGN
## Synonym: trans face
## Synonym: trans Golgi network

4 On-line vs. off-line data

It is possible to observe different results with rols and GO.db, as a result of the different ways they access the data. rols or biomaRt perform direct online queries, while GO.db and other annotation packages use database snapshot that are updated every release.

Both approaches have advantages. While online queries allow to obtain the latest up-to-date information, such approaches rely on network availability and quality. If reproducibility is a major issue, the version of the database to be queried can easily be controlled with off-line approaches. In the case of rols, although the load date of a specific ontology can be queried with olsVersion, it is not possible to query a specific version of an ontology.

5 Changes in version 2.0

rols 2.0 has substantially changed. While the table below shows some correspondence between the old and new interface, this is not always the case. The new interface relies on the Ontology/Ontologies, Term/Terms and OlsSearch classes, that need to be instantiated and can then be queried, as described above.

version < 1.99 version >= 1.99
ontologyLoadDate olsLoaded and olsUpdated
ontologyNames Ontologies
olsVersion olsVersion
allIds terms
isIdObsolete isObsolete
rootId olsRoot
olsQuery OlsSearch and olsSearch

Not all functionality is currently available. If there is anything that you need but not available in the new version, please contact the maintained by opening an issue on the package development site.

6 CVParams

The CVParam class is used to handle controlled vocabulary. It can be used for user-defined parameters

CVParam(name = "A user param", value = "the value")
## [, , A user param, the value]

or official controlled vocabulary (which triggers a query to the OLS service)

CVParam(label = "MS", accession = "MS:1000073")
## [MS, MS:1000073, electrospray ionization, ]
CVParam(label = "MS", name ="electrospray ionization")
## [MS, MS:1000073, electrospray ionization, ]
CVParam(label = "MS", name ="ESI") ## using a synonym
## [MS, MS:1000073, ESI, ]

See ?CVParam for more details and examples.

7 Session information

## R version 3.5.2 (2018-12-20)
## Platform: x86_64-pc-linux-gnu (64-bit)
## Running under: Ubuntu 16.04.5 LTS
## 
## Matrix products: default
## BLAS: /home/biocbuild/bbs-3.8-bioc/R/lib/libRblas.so
## LAPACK: /home/biocbuild/bbs-3.8-bioc/R/lib/libRlapack.so
## 
## attached base packages:
## [1] parallel  stats4    stats     graphics  grDevices utils     datasets 
## [8] methods   base     
## 
## other attached packages:
## [1] DT_0.5               rols_2.10.1          GO.db_3.7.0         
## [4] AnnotationDbi_1.44.0 IRanges_2.16.0       S4Vectors_0.20.1    
## [7] Biobase_2.42.0       BiocGenerics_0.28.0  BiocStyle_2.10.0    
## 
## loaded via a namespace (and not attached):
##  [1] Rcpp_1.0.0         later_0.7.5        compiler_3.5.2    
##  [4] BiocManager_1.30.4 prettyunits_1.0.2  tools_3.5.2       
##  [7] progress_1.2.0     digest_0.6.18      bit_1.1-14        
## [10] jsonlite_1.6       RSQLite_2.1.1      evaluate_0.12     
## [13] memoise_1.1.0      pkgconfig_2.0.2    rlang_0.3.0.1     
## [16] shiny_1.2.0        DBI_1.0.0          crosstalk_1.0.0   
## [19] curl_3.2           yaml_2.2.0         xfun_0.4          
## [22] stringr_1.3.1      httr_1.4.0         knitr_1.21        
## [25] htmlwidgets_1.3    hms_0.4.2          bit64_0.9-7       
## [28] R6_2.3.0           rmarkdown_1.11     bookdown_0.9      
## [31] blob_1.1.1         magrittr_1.5       promises_1.0.1    
## [34] htmltools_0.3.6    assertthat_0.2.0   xtable_1.8-3      
## [37] mime_0.6           httpuv_1.4.5.1     stringi_1.2.4     
## [40] crayon_1.3.4