contiBAIT v1.1.3 ============== Bug fixes: -Prevented bug where makeChrTable was creating GRanges starting at nucleotide 0 rather than 1 -Fixed bug that caused error when 'chrom' option was used in BAIT.R -Changed ideogramPlot to be more stable, quicker, no longer generate warnings. contiBAIT v1.1.2 ============== Bug fixes: -Prevented bug where ContigOrdering elements were slow to open due to computationally expensive sapply function in the definition contiBAIT v1.1.1 ============== Bug fixes: -Changed version number to align with Bioconductor contiBAIT v0.99.7 ============== Bug fixes: -prevented rare error where strandSeqFreqTable was crashing when there were no reads for all contigs Major changes: -Added table output of inversion/chimera statistics from locateMisorients -Made method for plotContigOrder and cleaned up code contiBAIT v0.99.6 ============== Bug fixes: Major changes: -Added function fixLinkageGroups. This iteratively looks through each ordered linkageGroup and assesses whether the contigs actually belong together based on similarity of adjacent contigs. For example, any adjacent contigs that are < 0.6 similar (this relatedCutOff is a user-definable option) are unlikely to actually be adjacent. This allows users to split groups that clustercContigs had erroneously merged -Added function mergeFlankedLGs. For organisms with large chromosomes, computing a consensus strand state for each linkage group is inefficient; the 'consensus' will be highly variable compared to the contigs at each extreme flank of a linkage group. This function takes an ordered linkage group, and computes a consensus for the extreme flank at each side of a linkage group. It then merges groups that clluster -To faciliate the above functions, orderAllLinkageGroups now outputs a list, with the contig order as the first element, and a series of strandStateMatrices as the second element. These strandStateMatrices are ordered, and parameters required for fixLinkageGroups and mergeFlankedContigs. The strandStateMatrices are themselves stored in a list, which constitutes a new class; StrandStateList Minor changes: -Fixed a few bugs where single-contig LGs were being ignored or throwing out errors in orderAllLinkageGroups -Added alreadyOrdered function to plotLGDistances, which prevents heatmap clustering and outputs in the order in which data is given. Will eventually remove the plotting function inherent in orderAllLinkageGroups contiBAIT v0.99.5 ============== Bug fixes: Major changes: -Added master function highlightAssemblyErrors for use in detecting misorientations and chimera Minor changes: -Added generic for thoroghBed -Fixed view error for LibraryGroupList with >10 elements -Removed empty/1 base elements from makeChrTable, that occasionally occurred while using a splitBy file contiBAIT v0.99.4 ============== Bug fixes: - fixed error where strandTable removal in preprocessStrandTable was resulting in empty matrix - fixed error where combineZeroDistances was creating an empty matrix that was too small for some data - fixed bugs in contiBAIT: a) bug where error would occur if verbose=FALSE but saveFiles=TRUE (because this.message only produced if verbose is TRUE) b) changed linkage.groups to linkage.merged the second time reorientLinkageGroups is called - Changed makeChrTable so it can now accept both a splitFile and a splitBy argument. Also will ensure that GRanges with meta columns won't interfere with splitting - plotContigOrder now defaults to all chromosomes unless 'lg' is specified - preprocessStrandTable has retired ignoreInternatlQual. To ignore quality libraries, just set lowQualityThreshold to NULL - strandSeqFreqTable now behaves differently if the filter file has strand information. Any elements in the filter object will have their reads flipped to reflect the orientation specified in this file. - replaced reorientLinkageGroup0s and mergeLinkageGroups into a combined function; reoientAndMergeLGs. New Functions: 1. findSimilarLibraries. Uses the clustering algorithm across libraries rather than contigs to find libraries where the distribution of directional reads are similar across a particular chromosome. These libraries can be merged into deeper files to look for chimerism using thoroughBed 2. thoroughBed. This function takes the list of lists from findSimilarLibraries and for each chromsome, merges similar libraries together. The output is a GRange object with merged reads for each chromosome 3. locateMisorients. This function takes the output of thoroghBed and using a circular binary segmenting algorithm to identify changes in strandState. The resulting file is of class ChrTable and includes directionality. As such, when fed into strandSeqFreqTable, regions of misorientations will be flipped, and chimeric sequence will be seperated. contiBAIT v0.99.3 ============== - Added some core R dependencies to make things happy with the new devel version of R - Shortened some lines of code contiBAIT v0.99.2 ============== (In response to first round of Bioconductor review). CLUSTERCONTIGS: -Now pre-creates the LinkageGroupList; more efficient than copy and replace MAKECHRTABLE: -Now returns GRanges object -All subsequent programs edited to take chrTable call as a GRanges -Divided chromosome table now included in makeChrTable, so dividedChrTable function removed -Ability to include additional split locations MERGELINKAGEGROUPS: -Now if clusNum is NULL, parallel clustering is not performed PLOTTING FUNCTIONS WITH GGPLOTS: -No longer return false positive issues with R CMD check MAPGAPFROMOVERLAP: -Retains data in GRanges, speeding up function GENERAL: -Simplified some longer one-liners. -Imported methods -All classes now have constructor functions contiBAIT v0.99.1 ============== IDEOGRAMPLOT: -fixed orientation issue. Now accepts OrientationTable to flip Watson and Crick reads MERGELINKAGEGROUPS: -fixed intermediate issue in mergeLinkageGroups where rownames were sometimes maintained and sometimes not after computeconsensus resulting in melt error. PREPROCESSSTRANDTABLE: - no longer looks for sex chromosomes. Was mostly pointless and didn't work well. Better method is to look for linkage groups with computeConsensus where consensus is mostly homozygous. REORIENTLINKAGEGROUPS -fixed bug where second iteration after mergeLinkageGroups was overwriting the OrientationTable. Now accepts a previousOrient argument if performing second reorientation. contiBAIT v0.99.0 (Submitted for BioC review 2016-01-20) ==============