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MaxContrastProjection

DOI: 10.18129/B9.bioc.MaxContrastProjection    

Perform a maximum contrast projection of 3D images along the z-dimension into 2D

Bioconductor version: Release (3.5)

A problem when recording 3D fluorescent microscopy images is how to properly present these results in 2D. Maximum intensity projections are a popular method to determine the focal plane of each pixel in the image. The problem with this approach, however, is that out-of-focus elements will still be visible, making edges and fine structures difficult to detect. This package aims to resolve this problem by using the contrast around a given pixel to determine the focal plane, allowing for a much cleaner structure detection than would be otherwise possible. For convenience, this package also contains functions to perform various other types of projections, including a maximum intensity projection.

Author: Jan Sauer, Bernd Fischer

Maintainer: Jan Sauer <jan.sauer at dkfz-heidelberg.de>

Citation (from within R, enter citation("MaxContrastProjection")):

Installation

To install this package, start R and enter:

## try http:// if https:// URLs are not supported
source("https://bioconductor.org/biocLite.R")
biocLite("MaxContrastProjection")

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("MaxContrastProjection")

 

PDF R Script MaxContrastProjection
PDF   Reference Manual
Text   NEWS

Details

biocViews CellBasedAssays, Preprocessing, Software, Visualization
Version 1.0.0
In Bioconductor since BioC 3.5 (R-3.4) (0.5 years)
License Artistic-2.0
Depends R (>= 3.4)
Imports EBImage, stats, methods
LinkingTo
Suggests knitr, BiocStyle, testthat
SystemRequirements GNU make
Enhances
URL
Depends On Me
Imports Me
Suggests Me
Build Report  

Package Archives

Follow Installation instructions to use this package in your R session.

Source Package MaxContrastProjection_1.0.0.tar.gz
Windows Binary MaxContrastProjection_1.0.0.zip
Mac OS X 10.11 (El Capitan) MaxContrastProjection_1.0.0.tgz
Source Repository git clone https://git.bioconductor.org/packages/MaxContrastProjection
Package Short Url http://bioconductor.org/packages/MaxContrastProjection/
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