Package version: specL 1.7.6

Contents

1 Introduction

Here we provide an experiment data package containing all the spread sheets used for figures and supplemental figures published in [1]. The raw instrument files are accessible for registered users through http://fgcz-bfabric.uzh.ch as project 1959. Raw LC-MS data from all five platforms were imported into Skyline 3.1 [2], processed, and are available though https://panoramaweb.org/labkey/MSQC1.url when published. This package contains data.frame objects exported by Skyline making the data available for the R world.

2 MSQC1 Peptide properties

2.1 Figure - reference L:H ratio versus the on-column amount

The scatter-plot displays the reference L:H ratio versus the on-column amount of heavy peptide of the MSQC1 peptide mix. Note, x and y axis are drawn in log scale.

3 Have fun with the data

show the dilution series

table(msqc1_dil$relative.amount)
## 
## 0.025  0.05   0.2     1     2     5 
##  2550  2550  2550  2550  2550  2550

show the peptide frequency

table(msqc1_dil$Peptide.Sequence)
## 
##    ADVTPADFSEWSK       ALIVLAHSER AVQQPDGLAVLGIFLK    DGLDAASYYAPVR 
##              378              864              828              378 
##    EGHLSPDIVAEQK       ESDTSYVSLK        FEDENFILK  FSTVAGESGSADTVR 
##             1170              864              684              864 
## GAGAFGYFEVTHDITK   GAGSSEPVTGLDAK        GGPFSDSYR     GTFIIDPAAVIR 
##              792              378              864              378 
##     GTFIIDPGGVIR       GYSIFSYATK   LFLQFGAQGSPFLK        LGGNEQVTR 
##              378              648              378              378 
##        NLSVEDAAR       SADFTNFDPR           TAENFR     TPVISGGPYEYR 
##              828              864              144              378 
##     TPVITGAPYEYR    VEATFGVDESNAK        VLDALQAIK        VSFELFADK 
##              378              378              864              864 
##       YILAGVENSK 
##              378

show ion types

table(msqc1_dil$Fragment.Ion)
## 
##              b8       precursor precursor [M+1] precursor [M+2] 
##              72            2106            2106            2106 
##             y10             y11             y12              y4 
##             720             144             144             396 
##              y5              y6              y7              y8 
##             990            1620            2106            1638 
##              y9 
##            1152

show instruments

table(msqc1_dil$instrument)
## 
##   QExactive QExactiveHF       QTRAP   TRIPLETOF  TSQVantage 
##        3996        4032        1242        4032        1998

4 Graphics

4.1 Figure - Sigma mix peptide level signals

Sigma mix peptide level signals - The graph displays the log2 L:H area ratios of eight technical replicates over 14 peptides from five MS platforms. The 14 panels were ordered by the on column amount of heavy peptide per vial (0.8, 4, 20, 40, 80, 200, 500, 1000 fmol). The black line indicates the theoretical L:H ratio as reported in the QC certificate by Sigma-Aldrich. In a perfect setting all data points would be located close to the black line indicating a perfect match between theoretical and measured log2 L:H ratios. The dark gray boxes correspond to a 0.5 and the light grey boxes to a deviation of 1 from the expected value (black line).

4.2 Figure - Volcano Plot

S <- .shape_for_volcano(S=msqc1_8rep, peptides)

msqc1:::.figure_setup()

xyplot(-log(p.value, 10) ~ log2FC | instrument, data=S, group=Peptide.Sequence,
       panel = function(...){
         panel.abline(v=c(-1,1), col='lightgray')
         panel.abline(h=-log(0.05,10), col='lightgray')
         panel.xyplot(...)
       },
       ylab='-log10 of p-value',
       xlab='log2 fold change',
       layout=c(1,5),
       auto.key = list(space = "right", points = TRUE, lines = FALSE, cex=1))

4.3 Figure - Ratio stability upon analyte dilution