Ribosome Profiling Data Analysis: from BAM to Data Representation and Interpretation


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Documentation for package ‘RiboProfiling’ version 1.34.0

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RiboProfiling-package RiboProfiling.
aroundPromoter Returns the flank size around the TSS for the x % CDSs
cdsPosTransc Per transcript relative position of start and end codons for dataset ctrlGAlignments
codonDataCtrl Codon frequency and coverage in ORFs on chromosome 1, for dataset ctrlGAlignments
codonIndexCovCtrl The read coverage for each codon in ORFs on chromosome 1, for dataset ctrlGAlignments
codonInfo Associates the read counts on codons with the codon type for each ORF.
codonPCA PCA graphs on codon coverage
countShiftReads Apply an offset on the read start along the transcript and returns the coverage on the 5pUTR, CDS, 3pUTR, as well as a matrix of codon coverage per ORF.
countsPlot Graphs of sample read counts (quality assesment)
ctrlGAlignments Ribosome profiling data on chr1 in human primary BJ fibroblasts control data: PMID: 23594524.
histMatchLength Histogram of match length distribution of reads.
orfRelativePos Relative position of the start and stop codon along the transcript
plotSummarizedCov Plots the summarized coverage in a specified range (e.g. around TSS) for the specified match sizes
printPCA Plots the PCA scatterplots produced by codonPCA function.
readStartCov Read start coverage around the TSS on the predifined CDSs
readsToStartOrEnd Reads in GAlignments converted to either Read Start (5') or End (3') Positions
RiboProfiling RiboProfiling.
riboSeqFromBAM Starting from a BAM file path: quality plots, shift ribosome position, coverage on multiple transcript features and on codons.