Generate customized protein database from NGS data, with a focus on RNA-Seq data, for proteomics search


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Documentation for package ‘customProDB’ version 1.42.1

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aaVariation get the functional consequencece of SNVs located in coding region
Bed2Range Generate a GRanges objects from BED file.
calculateRPKM Caculate RPKM for each transcripts based on exon read counts.
easyRun An integrated function to generate customized protein database for a single sample
easyRun_mul An integrated function to generate consensus protein database from multiple samples
InputVcf Generate a list of GRanges objects from a VCF file.
JunctionType Annotates the junctions in a bed file.
Multiple_VCF Generate shared variation dataset from multiple VCF files
Outputaberrant generate FASTA file containing short INDEL
OutputNovelJun generate peptide FASTA file that contains novel junctions.
Outputproseq output FASTA format file contains proteins that have expression level above the cutoff
OutputsharedPro Output the sequences of proteins with high expressions in multiple samples.
OutputVarprocodingseq Output the variant(SNVs) protein coding sequences
OutputVarproseq Output the variant(SNVs) protein sequences into FASTA format
OutputVarproseq_single Output the variant(SNVs) protein sequences into FASTA format
Positionincoding Find the position in coding sequence for each variation.
PrepareAnnotationEnsembl prepare annotation from ENSEMBL
PrepareAnnotationRefseq prepare annotation for Refseq
SharedJunc Generate shared junctions dataset from multiple BED files
Varlocation Annotates the variations with genomic location.