Sequence-based TF affinity scoring can be conducted with the FIMO suite, see @Sonawane2017. We have serialized an object with references to FIMO outputs for 16 TFs.
## GenomicFiles object with 1 ranges and 16 files:
## files: M0635_1.02sort.bed.gz, M3433_1.02sort.bed.gz, ..., M6159_1.02sort.bed.gz, M6497_1.02sort.bed.gz
## detail: use files(), rowRanges(), colData(), ...While the token bed is used in the filenames, the files are
not actually bed format!
scanTabixWe can use reduceByRange to import selected scans.
if (.Platform$OS.type != "windows") {
si = TFutils::seqinfo_hg19_chr17
myg = GRanges("chr17", IRanges(38.07e6,38.09e6), seqinfo=si)
colnames(fimo16) = fimo16$HGNC
lk2 = reduceByRange(fimo16[, c("POU2F1", "VDR")],
MAP=function(r,f) scanTabix(f, param=r))
str(lk2)
}## List of 1
## $ :List of 2
## ..$ POU2F1:List of 1
## .. ..$ chr17:38077000-38084000: chr [1:12] "chr17\t38077313\t38077331\tchr17:38077313-38077331\t2.15306\t+\t0.000352" "chr17\t38078549\t38078567\tchr17:38078549-38078567\t0.102041\t-\t0.000634" "chr17\t38078556\t38078574\tchr17:38078556-38078574\t-0.0408163\t-\t0.00066" "chr17\t38080045\t38080063\tchr17:38080045-38080063\t1.66327\t-\t0.000407" ...
## ..$ VDR :List of 1
## .. ..$ chr17:38077000-38084000: chr [1:18] "chr17\t38077445\t38077460\tchr17:38077445-38077460\t-1.9899\t-\t0.000666" "chr17\t38078536\t38078551\tchr17:38078536-38078551\t-2.66667\t-\t0.0008" "chr17\t38078574\t38078589\tchr17:38078574-38078589\t1.65657\t+\t0.000235" "chr17\t38078796\t38078811\tchr17:38078796-38078811\t2.07071\t-\t0.000207" ...This result can be massaged into a GRanges or other desirable structure.
fimo_granges takes care of this.
#fimo_ranges = function(gf, query) { # prototypical code
# rowRanges(gf) = query
# ans = reduceByRange(gf, MAP=function(r,f) scanTabix(f, param=r))
# ans = unlist(ans, recursive=FALSE) # drop top list structure
# tabs = lapply(ans, lapply, function(x) {
# con = textConnection(x)
# on.exit(close(con))
# dtf = read.delim(con, h=FALSE, stringsAsFactors=FALSE, sep="\t")
# colnames(dtf) = c("chr", "start", "end", "rname", "score", "dir", "pval")
# ans = with(dtf, GRanges(seqnames=chr, IRanges(start, end),
# rname=rname, score=score, dir=dir, pval=pval))
# ans
# })
# GRangesList(unlist(tabs, recursive=FALSE))
#}
if (.Platform$OS.type != "windows") {
rr = fimo_granges(fimo16[, c("POU2F1", "VDR")], myg)
rr
}## $POU2F1
## $POU2F1$`chr17:38070000-38090000`
## GRanges object with 76 ranges and 4 metadata columns:
## seqnames ranges strand | rname score
## <Rle> <IRanges> <Rle> | <character> <numeric>
## [1] chr17 38070239-38070257 * | chr17:38070239-38070.. -1.5408200
## [2] chr17 38070579-38070597 * | chr17:38070579-38070.. -0.9693880
## [3] chr17 38070851-38070869 * | chr17:38070851-38070.. 0.1224490
## [4] chr17 38071025-38071043 * | chr17:38071025-38071.. 0.0918367
## [5] chr17 38071253-38071271 * | chr17:38071253-38071.. 3.6734700
## ... ... ... ... . ... ...
## [72] chr17 38088602-38088620 * | chr17:38088602-38088.. 4.06122
## [73] chr17 38088637-38088655 * | chr17:38088637-38088.. 11.69390
## [74] chr17 38089141-38089159 * | chr17:38089141-38089.. 13.18370
## [75] chr17 38089439-38089457 * | chr17:38089439-38089.. -1.35714
## [76] chr17 38089822-38089840 * | chr17:38089822-38089.. 3.67347
## dir pval
## <character> <numeric>
## [1] + 0.000989
## [2] - 0.000849
## [3] - 0.000631
## [4] - 0.000636
## [5] + 0.000222
## ... ... ...
## [72] + 1.98e-04
## [73] - 1.32e-05
## [74] - 7.09e-06
## [75] - 9.42e-04
## [76] - 2.22e-04
## -------
## seqinfo: 1 sequence from hg19 genome
##
##
## $VDR
## $VDR$`chr17:38070000-38090000`
## GRanges object with 40 ranges and 4 metadata columns:
## seqnames ranges strand | rname score
## <Rle> <IRanges> <Rle> | <character> <numeric>
## [1] chr17 38070016-38070031 * | chr17:38070016-38070.. 0.0505051
## [2] chr17 38070387-38070402 * | chr17:38070387-38070.. -3.3838400
## [3] chr17 38070925-38070940 * | chr17:38070925-38070.. 5.7171700
## [4] chr17 38071183-38071198 * | chr17:38071183-38071.. -0.6767680
## [5] chr17 38072289-38072304 * | chr17:38072289-38072.. -0.3333330
## ... ... ... ... . ... ...
## [36] chr17 38086915-38086930 * | chr17:38086915-38086.. -0.353535
## [37] chr17 38087304-38087319 * | chr17:38087304-38087.. 0.101010
## [38] chr17 38087866-38087881 * | chr17:38087866-38087.. 5.343430
## [39] chr17 38088893-38088908 * | chr17:38088893-38088.. -0.111111
## [40] chr17 38089214-38089229 * | chr17:38089214-38089.. 1.101010
## dir pval
## <character> <numeric>
## [1] + 3.77e-04
## [2] + 9.66e-04
## [3] - 6.44e-05
## [4] + 4.63e-04
## [5] - 4.21e-04
## ... ... ...
## [36] - 0.000423
## [37] - 0.000371
## [38] + 0.000073
## [39] - 0.000395
## [40] - 0.000277
## -------
## seqinfo: 1 sequence from hg19 genome## R version 4.2.1 (2022-06-23)
## Platform: x86_64-pc-linux-gnu (64-bit)
## Running under: Ubuntu 20.04.5 LTS
##
## Matrix products: default
## BLAS: /home/biocbuild/bbs-3.16-bioc/R/lib/libRblas.so
## LAPACK: /home/biocbuild/bbs-3.16-bioc/R/lib/libRlapack.so
##
## locale:
## [1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C
## [3] LC_TIME=en_GB LC_COLLATE=C
## [5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8
## [7] LC_PAPER=en_US.UTF-8 LC_NAME=C
## [9] LC_ADDRESS=C LC_TELEPHONE=C
## [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
##
## attached base packages:
## [1] grid stats4 stats graphics grDevices utils datasets
## [8] methods base
##
## other attached packages:
## [1] UpSetR_1.4.0 magrittr_2.0.3
## [3] dplyr_1.0.10 gwascat_2.30.0
## [5] GSEABase_1.60.0 graph_1.76.0
## [7] annotate_1.76.0 XML_3.99-0.12
## [9] png_0.1-7 ggplot2_3.3.6
## [11] knitr_1.40 data.table_1.14.4
## [13] GO.db_3.16.0 GenomicFiles_1.34.0
## [15] rtracklayer_1.58.0 Rsamtools_2.14.0
## [17] Biostrings_2.66.0 XVector_0.38.0
## [19] BiocParallel_1.32.0 SummarizedExperiment_1.28.0
## [21] GenomicRanges_1.50.0 GenomeInfoDb_1.34.0
## [23] MatrixGenerics_1.10.0 matrixStats_0.62.0
## [25] org.Hs.eg.db_3.16.0 AnnotationDbi_1.60.0
## [27] IRanges_2.32.0 S4Vectors_0.36.0
## [29] Biobase_2.58.0 BiocGenerics_0.44.0
## [31] TFutils_1.18.0 BiocStyle_2.26.0
##
## loaded via a namespace (and not attached):
## [1] colorspace_2.0-3 rjson_0.2.21 ellipsis_0.3.2
## [4] farver_2.1.1 bit64_4.0.5 fansi_1.0.3
## [7] xml2_1.3.3 splines_4.2.1 codetools_0.2-18
## [10] snpStats_1.48.0 cachem_1.0.6 jsonlite_1.8.3
## [13] dbplyr_2.2.1 shiny_1.7.3 BiocManager_1.30.19
## [16] readr_2.1.3 compiler_4.2.1 httr_1.4.4
## [19] assertthat_0.2.1 Matrix_1.5-1 fastmap_1.1.0
## [22] cli_3.4.1 later_1.3.0 htmltools_0.5.3
## [25] prettyunits_1.1.1 tools_4.2.1 gtable_0.3.1
## [28] glue_1.6.2 GenomeInfoDbData_1.2.9 rappdirs_0.3.3
## [31] Rcpp_1.0.9 cellranger_1.1.0 jquerylib_0.1.4
## [34] vctrs_0.5.0 xfun_0.34 stringr_1.4.1
## [37] mime_0.12 miniUI_0.1.1.1 lifecycle_1.0.3
## [40] restfulr_0.0.15 zlibbioc_1.44.0 scales_1.2.1
## [43] BSgenome_1.66.0 VariantAnnotation_1.44.0 hms_1.1.2
## [46] promises_1.2.0.1 parallel_4.2.1 yaml_2.3.6
## [49] curl_4.3.3 gridExtra_2.3 memoise_2.0.1
## [52] sass_0.4.2 biomaRt_2.54.0 stringi_1.7.8
## [55] RSQLite_2.2.18 highr_0.9 BiocIO_1.8.0
## [58] GenomicFeatures_1.50.0 filelock_1.0.2 rlang_1.0.6
## [61] pkgconfig_2.0.3 bitops_1.0-7 evaluate_0.17
## [64] lattice_0.20-45 labeling_0.4.2 GenomicAlignments_1.34.0
## [67] bit_4.0.4 tidyselect_1.2.0 bookdown_0.29
## [70] plyr_1.8.7 R6_2.5.1 generics_0.1.3
## [73] DelayedArray_0.24.0 DBI_1.1.3 pillar_1.8.1
## [76] withr_2.5.0 survival_3.4-0 KEGGREST_1.38.0
## [79] RCurl_1.98-1.9 tibble_3.1.8 crayon_1.5.2
## [82] utf8_1.2.2 BiocFileCache_2.6.0 tzdb_0.3.0
## [85] rmarkdown_2.17 progress_1.2.2 readxl_1.4.1
## [88] blob_1.2.3 digest_0.6.30 xtable_1.8-4
## [91] httpuv_1.6.6 munsell_0.5.0 bslib_0.4.0