cellCellSimulate
functionscTensor 2.8.0
Here, we explain the way to generate CCI simulation data.
scTensor has a function cellCellSimulate
to generate the simulation data.
The simplest way to generate such data is cellCellSimulate
with default parameters.
suppressPackageStartupMessages(library("scTensor"))
sim <- cellCellSimulate()
## Getting the values of params...
## Setting random seed...
## Generating simulation data...
## Done!
This function internally generate the parameter sets by newCCSParams
,
and the values of the parameter can be changed, and specified as the input of cellCellSimulate
by users as follows.
# Default parameters
params <- newCCSParams()
str(params)
## Formal class 'CCSParams' [package "scTensor"] with 5 slots
## ..@ nGene : num 1000
## ..@ nCell : num [1:3] 50 50 50
## ..@ cciInfo:List of 4
## .. ..$ nPair: num 500
## .. ..$ CCI1 :List of 4
## .. .. ..$ LPattern: num [1:3] 1 0 0
## .. .. ..$ RPattern: num [1:3] 0 1 0
## .. .. ..$ nGene : num 50
## .. .. ..$ fc : chr "E10"
## .. ..$ CCI2 :List of 4
## .. .. ..$ LPattern: num [1:3] 0 1 0
## .. .. ..$ RPattern: num [1:3] 0 0 1
## .. .. ..$ nGene : num 50
## .. .. ..$ fc : chr "E10"
## .. ..$ CCI3 :List of 4
## .. .. ..$ LPattern: num [1:3] 0 0 1
## .. .. ..$ RPattern: num [1:3] 1 0 0
## .. .. ..$ nGene : num 50
## .. .. ..$ fc : chr "E10"
## ..@ lambda : num 1
## ..@ seed : num 1234
# Setting different parameters
# No. of genes : 1000
setParam(params, "nGene") <- 1000
# 3 cell types, 20 cells in each cell type
setParam(params, "nCell") <- c(20, 20, 20)
# Setting for Ligand-Receptor pair list
setParam(params, "cciInfo") <- list(
nPair=500, # Total number of L-R pairs
# 1st CCI
CCI1=list(
LPattern=c(1,0,0), # Only 1st cell type has this pattern
RPattern=c(0,1,0), # Only 2nd cell type has this pattern
nGene=50, # 50 pairs are generated as CCI1
fc="E10"), # Degree of differential expression (Fold Change)
# 2nd CCI
CCI2=list(
LPattern=c(0,1,0),
RPattern=c(0,0,1),
nGene=30,
fc="E100")
)
# Degree of Dropout
setParam(params, "lambda") <- 10
# Random number seed
setParam(params, "seed") <- 123
# Simulation data
sim <- cellCellSimulate(params)
## Getting the values of params...
## Setting random seed...
## Generating simulation data...
## Done!
The output object sim has some attributes as follows.
Firstly, sim$input contains a synthetic gene expression matrix. The size can be changed by nGene and nCell parameters described above.
dim(sim$input)
## [1] 1000 60
sim$input[1:2,1:3]
## Cell1 Cell2 Cell3
## Gene1 9105 2 0
## Gene2 4 37 850
Next, sim$LR contains a ligand-receptor (L-R) pair list. The size can be changed by nPair parameter of cciInfo, and the differentially expressed (DE) L-R pairs are saved in the upper side of this matrix. Here, two DE L-R patterns are specified as cciInfo, and each number of pairs is 50 and 30, respectively.
dim(sim$LR)
## [1] 500 2
sim$LR[1:10,]
## GENEID_L GENEID_R
## 1 Gene1 Gene81
## 2 Gene2 Gene82
## 3 Gene3 Gene83
## 4 Gene4 Gene84
## 5 Gene5 Gene85
## 6 Gene6 Gene86
## 7 Gene7 Gene87
## 8 Gene8 Gene88
## 9 Gene9 Gene89
## 10 Gene10 Gene90
sim$LR[46:55,]
## GENEID_L GENEID_R
## 46 Gene46 Gene126
## 47 Gene47 Gene127
## 48 Gene48 Gene128
## 49 Gene49 Gene129
## 50 Gene50 Gene130
## 51 Gene51 Gene131
## 52 Gene52 Gene132
## 53 Gene53 Gene133
## 54 Gene54 Gene134
## 55 Gene55 Gene135
sim$LR[491:500,]
## GENEID_L GENEID_R
## 491 Gene571 Gene991
## 492 Gene572 Gene992
## 493 Gene573 Gene993
## 494 Gene574 Gene994
## 495 Gene575 Gene995
## 496 Gene576 Gene996
## 497 Gene577 Gene997
## 498 Gene578 Gene998
## 499 Gene579 Gene999
## 500 Gene580 Gene1000
Finally, sim$celltypes contains a cell type vector. Since nCell is specified as “c(20, 20, 20)” described above, three cell types are generated.
length(sim$celltypes)
## [1] 60
head(sim$celltypes)
## Celltype1 Celltype1 Celltype1 Celltype1 Celltype1 Celltype1
## "Cell1" "Cell2" "Cell3" "Cell4" "Cell5" "Cell6"
table(names(sim$celltypes))
##
## Celltype1 Celltype2 Celltype3
## 20 20 20
## R version 4.2.1 Patched (2022-07-09 r82577)
## Platform: x86_64-apple-darwin17.0 (64-bit)
## Running under: macOS Big Sur ... 10.16
##
## Matrix products: default
## BLAS: /Library/Frameworks/R.framework/Versions/4.2/Resources/lib/libRblas.0.dylib
## LAPACK: /Library/Frameworks/R.framework/Versions/4.2/Resources/lib/libRlapack.dylib
##
## locale:
## [1] C/en_US.UTF-8/en_US.UTF-8/C/en_GB/en_US.UTF-8
##
## attached base packages:
## [1] stats4 stats graphics grDevices utils datasets methods
## [8] base
##
## other attached packages:
## [1] scTGIF_1.12.0
## [2] Homo.sapiens_1.3.1
## [3] TxDb.Hsapiens.UCSC.hg19.knownGene_3.2.2
## [4] org.Hs.eg.db_3.16.0
## [5] GO.db_3.16.0
## [6] OrganismDbi_1.40.0
## [7] GenomicFeatures_1.50.0
## [8] AnnotationDbi_1.60.0
## [9] SingleCellExperiment_1.20.0
## [10] SummarizedExperiment_1.28.0
## [11] Biobase_2.58.0
## [12] GenomicRanges_1.50.0
## [13] GenomeInfoDb_1.34.0
## [14] IRanges_2.32.0
## [15] S4Vectors_0.36.0
## [16] MatrixGenerics_1.10.0
## [17] matrixStats_0.62.0
## [18] scTensor_2.8.0
## [19] RSQLite_2.2.18
## [20] LRBaseDbi_2.8.0
## [21] AnnotationHub_3.6.0
## [22] BiocFileCache_2.6.0
## [23] dbplyr_2.2.1
## [24] BiocGenerics_0.44.0
## [25] BiocStyle_2.26.0
##
## loaded via a namespace (and not attached):
## [1] ica_1.0-3 Rsamtools_2.14.0
## [3] foreach_1.5.2 lmtest_0.9-40
## [5] crayon_1.5.2 spatstat.core_2.4-4
## [7] MASS_7.3-58.1 nlme_3.1-160
## [9] backports_1.4.1 GOSemSim_2.24.0
## [11] MeSHDbi_1.34.0 rlang_1.0.6
## [13] XVector_0.38.0 HDO.db_0.99.1
## [15] ROCR_1.0-11 irlba_2.3.5.1
## [17] nnTensor_1.1.8 ca_0.71.1
## [19] filelock_1.0.2 GOstats_2.64.0
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