1 Installation and getting started

The crisprVerse is a collection of packages for CRISPR guide RNA (gRNA) design that can easily be installed with the crisprVerse package. This provides a convenient way of downloading and installing all crisprVerse packages with a single R command.

The package can be installed from the Bioconductor devel branch using the following commands in an R session:

if (!requireNamespace("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install(version="devel")
BiocManager::install("crisprVerse")

The core crisprVerse includes the packages that are commonly used for gRNA design, and are attached when you attach the crisprVerse package:

library(crisprVerse)

You can check that all crisprVerse packages are up-to-date with the function crisprVerse_update().

2 Components

The following packages are installed and loaded with the crisprVerse package:

  • crisprBase to specify and manipulate CRISPR nucleases.
  • crisprBowtie to perform gRNA spacer sequence alignment with Bowtie.
  • crisprScore to annotate gRNAs with on-target and off-target scores.
  • crisprDesign to design and manipulate gRNAs with GuideSet objects.
  • crisprViz to visualize gRNAs.

3 Reproducibility

sessionInfo()
## R version 4.2.1 (2022-06-23)
## Platform: aarch64-apple-darwin20 (64-bit)
## Running under: macOS Ventura 13.0
## 
## Matrix products: default
## BLAS:   /Library/Frameworks/R.framework/Versions/4.2-arm64/Resources/lib/libRblas.0.dylib
## LAPACK: /Library/Frameworks/R.framework/Versions/4.2-arm64/Resources/lib/libRlapack.dylib
## 
## locale:
## [1] C/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8
## 
## attached base packages:
## [1] stats     graphics  grDevices utils     datasets  methods   base     
## 
## other attached packages:
##  [1] crisprViz_1.0.0       crisprDesign_1.0.0    crisprScore_1.2.0    
##  [4] crisprScoreData_1.1.3 ExperimentHub_2.6.0   AnnotationHub_3.6.0  
##  [7] BiocFileCache_2.6.0   dbplyr_2.2.1          BiocGenerics_0.44.0  
## [10] crisprBowtie_1.2.0    crisprBase_1.2.0      crisprVerse_1.0.0    
## [13] BiocStyle_2.26.0     
## 
## loaded via a namespace (and not attached):
##   [1] backports_1.4.1               Hmisc_4.7-0                  
##   [3] lazyeval_0.2.2                splines_4.2.1                
##   [5] BiocParallel_1.32.1           GenomeInfoDb_1.34.2          
##   [7] ggplot2_3.3.6                 digest_0.6.29                
##   [9] ensembldb_2.22.0              htmltools_0.5.2              
##  [11] fansi_1.0.3                   checkmate_2.1.0              
##  [13] magrittr_2.0.3                memoise_2.0.1                
##  [15] BSgenome_1.66.1               cluster_2.1.3                
##  [17] tzdb_0.3.0                    Biostrings_2.66.0            
##  [19] readr_2.1.2                   matrixStats_0.62.0           
##  [21] prettyunits_1.1.1             jpeg_0.1-9                   
##  [23] colorspace_2.0-3              blob_1.2.3                   
##  [25] rappdirs_0.3.3                xfun_0.31                    
##  [27] dplyr_1.0.9                   crayon_1.5.1                 
##  [29] RCurl_1.98-1.7                jsonlite_1.8.0               
##  [31] survival_3.3-1                VariantAnnotation_1.44.0     
##  [33] glue_1.6.2                    gtable_0.3.0                 
##  [35] zlibbioc_1.44.0               XVector_0.38.0               
##  [37] DelayedArray_0.24.0           scales_1.2.0                 
##  [39] DBI_1.1.3                     Rcpp_1.0.9                   
##  [41] htmlTable_2.4.1               xtable_1.8-4                 
##  [43] progress_1.2.2                reticulate_1.25              
##  [45] foreign_0.8-82                bit_4.0.4                    
##  [47] Formula_1.2-4                 stats4_4.2.1                 
##  [49] htmlwidgets_1.5.4             httr_1.4.3                   
##  [51] dir.expiry_1.6.0              RColorBrewer_1.1-3           
##  [53] ellipsis_0.3.2                pkgconfig_2.0.3              
##  [55] XML_3.99-0.10                 nnet_7.3-17                  
##  [57] Gviz_1.42.0                   sass_0.4.1                   
##  [59] deldir_1.0-6                  utf8_1.2.2                   
##  [61] tidyselect_1.1.2              rlang_1.0.4                  
##  [63] later_1.3.0                   AnnotationDbi_1.60.0         
##  [65] munsell_0.5.0                 BiocVersion_3.16.0           
##  [67] tools_4.2.1                   cachem_1.0.6                 
##  [69] cli_3.3.0                     generics_0.1.3               
##  [71] RSQLite_2.2.14                evaluate_0.15                
##  [73] stringr_1.4.0                 fastmap_1.1.0                
##  [75] yaml_2.3.5                    knitr_1.39                   
##  [77] bit64_4.0.5                   purrr_0.3.4                  
##  [79] randomForest_4.7-1.1          AnnotationFilter_1.22.0      
##  [81] KEGGREST_1.38.0               Rbowtie_1.38.0               
##  [83] mime_0.12                     xml2_1.3.3                   
##  [85] biomaRt_2.54.0                rstudioapi_0.13              
##  [87] compiler_4.2.1                filelock_1.0.2               
##  [89] curl_4.3.2                    png_0.1-7                    
##  [91] interactiveDisplayBase_1.36.0 tibble_3.1.7                 
##  [93] bslib_0.3.1                   stringi_1.7.8                
##  [95] basilisk.utils_1.10.0         GenomicFeatures_1.50.2       
##  [97] lattice_0.20-45               ProtGenerics_1.30.0          
##  [99] Matrix_1.4-1                  vctrs_0.4.1                  
## [101] pillar_1.7.0                  lifecycle_1.0.1              
## [103] BiocManager_1.30.18           jquerylib_0.1.4              
## [105] data.table_1.14.2             bitops_1.0-7                 
## [107] httpuv_1.6.5                  rtracklayer_1.58.0           
## [109] GenomicRanges_1.50.1          R6_2.5.1                     
## [111] BiocIO_1.8.0                  latticeExtra_0.6-30          
## [113] bookdown_0.27                 promises_1.2.0.1             
## [115] gridExtra_2.3                 IRanges_2.32.0               
## [117] codetools_0.2-18              dichromat_2.0-0.1            
## [119] assertthat_0.2.1              SummarizedExperiment_1.28.0  
## [121] rjson_0.2.21                  GenomicAlignments_1.34.0     
## [123] Rsamtools_2.14.0              S4Vectors_0.36.0             
## [125] GenomeInfoDbData_1.2.8        parallel_4.2.1               
## [127] hms_1.1.1                     rpart_4.1.16                 
## [129] grid_4.2.1                    basilisk_1.10.1              
## [131] rmarkdown_2.14                MatrixGenerics_1.10.0        
## [133] biovizBase_1.46.0             Biobase_2.58.0               
## [135] shiny_1.7.1                   base64enc_0.1-3              
## [137] interp_1.1-3                  restfulr_0.0.15