pipeline for single cell RNA-seq data analysis


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Documentation for package ‘scPipe’ version 1.14.0

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scPipe-package scPipe - single cell RNA-seq pipeline
anno_import Import gene annotation
anno_to_saf Convert annotation from GenomicRanges to Simple Annotation Format (SAF)
calculate_QC_metrics Calcuate QC metrics from gene count matrix
cell_barcode_matching cell barcode demultiplex statistics for a small sample scRNA-seq dataset to demonstrate capabilities of scPipe
convert_geneid convert the gene ids of a SingleCellExperiment object
create_processed_report create_processed_report
create_report create_report
create_sce_by_dir create a SingleCellExperiment object from data folder generated by preprocessing step
demultiplex_info demultiplex_info
demultiplex_info-method demultiplex_info
demultiplex_info.sce demultiplex_info
demultiplex_info<- demultiplex_info
demultiplex_info<--method demultiplex_info
detect_outlier Detect outliers based on QC metrics
gene_id_type Get or set 'gene_id_type' from a SingleCellExperiment object
gene_id_type-method Get or set 'gene_id_type' from a SingleCellExperiment object
gene_id_type.sce Get or set 'gene_id_type' from a SingleCellExperiment object
gene_id_type<- Get or set 'gene_id_type' from a SingleCellExperiment object
gene_id_type<--method Get or set 'gene_id_type' from a SingleCellExperiment object
get_ercc_anno Get ERCC annotation table
get_genes_by_GO Get genes related to certain GO terms from biomart database
get_read_str Get read structure for particular scRNA-seq protocol
organism Get or set 'organism' from a SingleCellExperiment object
organism-method Get or set 'organism' from a SingleCellExperiment object
organism.sce Get or set 'organism' from a SingleCellExperiment object
organism<--method Get or set 'organism' from a SingleCellExperiment object
plot_demultiplex plot_demultiplex
plot_mapping Plot mapping statistics for 'SingleCellExperiment' object.
plot_QC_pairs Plot GGAlly pairs plot of QC statistics from 'SingleCellExperiment' object
plot_UMI_dup Plot UMI duplication frequency
QC_metrics Get or set quality control metrics in a SingleCellExperiment object
QC_metrics-method Get or set quality control metrics in a SingleCellExperiment object
QC_metrics.sce Get or set quality control metrics in a SingleCellExperiment object
QC_metrics<- Get or set quality control metrics in a SingleCellExperiment object
QC_metrics<--method Get or set quality control metrics in a SingleCellExperiment object
remove_outliers Remove outliers in 'SingleCellExperiment'
scPipe scPipe - single cell RNA-seq pipeline
sc_correct_bam_bc sc_correct_bam_bc
sc_count_aligned_bam sc_count_aligned_bam
sc_demultiplex sc_demultiplex
sc_demultiplex_and_count sc_demultiplex_and_count
sc_detect_bc sc_detect_bc
sc_exon_mapping sc_exon_mapping
sc_gene_counting sc_gene_counting
sc_sample_data a small sample scRNA-seq counts dataset to demonstrate capabilities of scPipe
sc_sample_qc quality control information for a small sample scRNA-seq dataset to demonstrate capabilities of scPipe.
sc_trim_barcode sc_trim_barcode
UMI_duplication UMI duplication statistics for a small sample scRNA-seq dataset to demonstrate capabilities of scPipe
UMI_dup_info Get or set UMI duplication results in a SingleCellExperiment object
UMI_dup_info-method Get or set UMI duplication results in a SingleCellExperiment object
UMI_dup_info.sce Get or set UMI duplication results in a SingleCellExperiment object
UMI_dup_info<- Get or set UMI duplication results in a SingleCellExperiment object
UMI_dup_info<--method Get or set UMI duplication results in a SingleCellExperiment object