To generate plate maps, WPM uses an algorithm inspired from the backtracking algorithm. More precisely, WPM loops on the following actions until all of the samples are given a correct location:
This process allows for an experimental design by block randomization.
There are two ways using the wpm
package:
Important: Even in case of command line use, we strongly recommend to read the section about the shiny app section, as this is where all terms and concepts are explained in detail.
Input Format | Command line | WPM app |
---|---|---|
CSV | yes | yes |
ExpressionSet | yes | no |
SummarizedExperiment | yes | no |
MSnSet | yes | no |
This tutorial explains how to use the Well Plate Maker package. Make sure you are using a recent R version (\(\geq 4.0.0\)). For Windows users who do not have the Edge browser, we recommend using the Chrome browser rather than Internet Explorer.
if (!requireNamespace("BiocManager", quietly = TRUE))
install.packages("BiocManager")
BiocManager::install("wpm")
Whether you use RStudio or simply work with an R console, the procedure remains the same to launch the shiny app:
library(wpm)
wpm()
If everything is in order, a new window will open in your default browser.
If not, find the line written in the R console: Listening on http://127.0.0.1:8000
, and paste the URL in your web browser.
WPM has 4 main tabs: Home, Parameters, Results ans Help.
Briefly presents the aim of the app, shows the last package version, explains how to cite us to support our work and gives the contact information.