EasyqpcR for low-throughput real-time quantitative PCR data analysis


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Documentation for package ‘EasyqpcR’ version 1.10.2

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EasyqpcR-package Functions to analyse real-time quantitative PCR data at IRTOMIT-INSERM U1082
badCt Evaluation of the qPCR technical replicates
calData Calculation of calibration factors
EasyqpcR Functions to analyse real-time quantitative PCR data at IRTOMIT-INSERM U1082
Efficiency_calculation Raw data for primer amplification efficiency calculation.
Gene_maximisation A data frame containing all the data from four different qPCR runs.
Gene_maximisation_cor A data frame containing all the data from four different qPCR runs.
nrmData Determination of the NF, RQ, NRQ, NRQ scaled to control and their SE and SD.
qPCR_run1 Raw data from the first qPCR run.
qPCR_run2 Raw data from the second qPCR run.
qPCR_run3 Raw data from the third qPCR run.
slope Function to calculate the amplification efficiency
totData Aggregation of qPCR biological replicates and data transformation