Analysis tools for single-cell expression experiments.


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Documentation for package ‘monocle’ version 1.2.0

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CellDataSet The CellDataSet class
CellDataSet-class The CellDataSet class
cellPairwiseDistances Retrieves a matrix capturing distances between each cell in the reduced-dimensionality space
cellPairwiseDistances<- Sets the matrix containing distances between each pair of cells used by Monocle during cell ordering. Not intended to be called directly.
clusterGenes Plots the minimum spanning tree on cells.
compareModels Compare model fits
detectGenes Sets the global expression detection threshold to be used with this CellDataSet. Counts how many cells each feature in a CellDataSet object that are detectably expressed above a minimum threshold. Also counts the number of genes above this threshold are detectable in each cell.
differentialGeneTest Tests each gene for differential expression as a function of progress through a biological process, or according to other covariates as specified.
fitModel Fits a model for each gene in a CellDataSet object.
minSpanningTree Retrieve the minimum spanning tree generated by Monocle during cell ordering.
minSpanningTree<- Sets the minimum spanning tree used by Monocle during cell ordering. Not intended to be called directly.
newCellDataSet Creates a new CellDateSet object.
orderCells Orders cells according to progress through a learned biological process.
plot_clusters Plots the minimum spanning tree on cells.
plot_genes_in_pseudotime Plots expression for one or more genes as a function of pseudotime
plot_genes_jitter Plots expression for one or more genes as a jittered, grouped points
plot_genes_positive_cells Plots the number of cells expressing one or more genes as a barplot
plot_spanning_tree Plots the minimum spanning tree on cells.
reducedDimA Get the weights needed to lift cells back to high dimensional expression space.
reducedDimA<- Get the weights needed to lift cells back to high dimensional expression space.
reducedDimS Retrieves the coordinates of each cell in the reduced-dimensionality space generated by calls to reduceDimension.
reducedDimS<- Set embedding coordinates of each cell in a CellDataSet.
reducedDimW Get the whitened expression values for a CellDataSet.
reducedDimW<- Get the whitened expression values for a CellDataSet.
reduceDimension Computes a projection of a CellDataSet object into a lower dimensional space
responseMatrix Response values
selectNegentropyGenes Filter genes with extremely high or low negentropy
setOrderingFilter Sets the features (e.g. genes) to be used for ordering cells in pseudotime.