SNPAssayR 1.0.1

• Initial release: offline CAPS, single-substitution site-creation dCAPS, conventional two-reaction ARMS and tetra-primer ARMS candidate design.
• Evaluate perfect-match primer thermodynamics, sequence compatibility and theoretical complete digests with explicit uncertainty and search limits.
• Provide source-linked coordinate and band schematics, within-method preference ranking, reproducible exports and an optional local Shiny interface.
• Apply tetra-primer product-size bounds to the control and both diagnostic products; retain separate broad pairwise risk screening.
• Include runnable public-API examples and regenerable computational references.
• Expand the source-verified enzyme panel to 20 distinct motifs, including MfeI-HF.
• Add an offline 550-bp synthetic teaching locus and practical-length workflows for CAPS, dCAPS, conventional ARMS and tetra-primer ARMS.
• Provide a version-specific software citation through citation(“SNPAssayR”).
• Record ICAR-Indian Institute of Rapeseed-Mustard Research (ICAR-IIRMR) as copyright holder while retaining the three authors and designated maintainer.